TY - JOUR
T1 - The zebrafish embryo as a model to quantify early inflammatory cell responses to biomaterials
AU - Zhang, Xiaolin
AU - Stockhammer, Oliver W.
AU - de Boer, Leonie
AU - Vischer, Norbert O. E.
AU - Spaink, Herman P.
AU - Grijpma, Dirk W.
AU - Zaat, Sebastian A. J.
PY - 2017
Y1 - 2017
N2 - To rapidly assess early inflammatory cell responses provoked by biomaterials in the full complexity of the living organism, we developed a zebrafish embryo model which allows real time analysis of these responses to biomaterial microspheres. Fluorescently labeled microspheres with different properties were injected into embryos of selected transgenic zebrafish lines expressing distinct fluorescent proteins in their neutrophils and macrophages. Recruitment of leukocytes and their interactions with microspheres were monitored using fluorescence microscopy. We developed a novel method using ImageJ and the plugin ObjectJ project file "Zebrafish-Immunotest" for rapid and semi-automated fluorescence quantification of the cellular responses. In the embryo model we observed an ordered inflammatory cell response to polystyrene and poly (e-caprolactone) microspheres, similar to that described for mammalian animal models. The responses were characterized by an early infiltration of neutrophils followed by macrophages, and subsequent differentially timed migration of these cells away from the microspheres. The size of microspheres (10 and 15 mm) did not influence the cellular responses. Poly (e-caprolactone) microspheres provoked a stronger infiltration of neutrophils and macrophages than polystyrene microspheres did. Our study shows the potential usefulness of zebrafish embryos for in vivo evaluation of biomaterial-associated inflammatory cell responses. VC 2017 Wiley Periodicals,
AB - To rapidly assess early inflammatory cell responses provoked by biomaterials in the full complexity of the living organism, we developed a zebrafish embryo model which allows real time analysis of these responses to biomaterial microspheres. Fluorescently labeled microspheres with different properties were injected into embryos of selected transgenic zebrafish lines expressing distinct fluorescent proteins in their neutrophils and macrophages. Recruitment of leukocytes and their interactions with microspheres were monitored using fluorescence microscopy. We developed a novel method using ImageJ and the plugin ObjectJ project file "Zebrafish-Immunotest" for rapid and semi-automated fluorescence quantification of the cellular responses. In the embryo model we observed an ordered inflammatory cell response to polystyrene and poly (e-caprolactone) microspheres, similar to that described for mammalian animal models. The responses were characterized by an early infiltration of neutrophils followed by macrophages, and subsequent differentially timed migration of these cells away from the microspheres. The size of microspheres (10 and 15 mm) did not influence the cellular responses. Poly (e-caprolactone) microspheres provoked a stronger infiltration of neutrophils and macrophages than polystyrene microspheres did. Our study shows the potential usefulness of zebrafish embryos for in vivo evaluation of biomaterial-associated inflammatory cell responses. VC 2017 Wiley Periodicals,
U2 - https://doi.org/10.1002/jbm.a.36110
DO - https://doi.org/10.1002/jbm.a.36110
M3 - Article
C2 - 28509403
SN - 1549-3296
VL - 105
SP - 2522
EP - 2532
JO - Journal of biomedical materials research. Part A
JF - Journal of biomedical materials research. Part A
IS - 9
ER -