TY - JOUR
T1 - Tracing goes viral: Viruses that introduce expression of fluorescent proteins in chemically-specific neurons
T2 - Viruses that introduce expression of fluorescent proteins in chemically-specific neurons
AU - Luchicchi, Antonio
AU - Pattij, Tommy
AU - Viaña, John Noel M.
AU - de Kloet, Sybren
AU - Marchant, Nathan
N1 - Funding Information: Confocal images and immunostaining was in part performed in the lab of Prof. Dr. Huibert D. Mansvelder (CNCR, VU Amsterdam) using funding coming from his personal grants. We wish to thank him for his generous financial support. This paper also draws on results from JNMV's internship at the CNCR, VU Amsterdam, which was supported by a NEURASMUS Erasmus Mundus scholarship . JNMV is currently supported by a postdoctoral fellowship from the ANU-CSIRO (Australian National University - Commonwealth Scientific and Industrial Research Organisation) Responsible Innovation collaboration. Publisher Copyright: © 2020 Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/15
Y1 - 2021/1/15
N2 - Over the last century, there has been great progress in understanding how the brain works. In particular, the last two decades have been crucial in gaining more awareness over the complex functioning of neurotransmitter systems. The use of viral vectors in neuroscience has been pivotal for such development. Exploiting the properties of viral particles, modifying them according to the research needs, and making them target chemically-specific neurons, techniques such as optogenetics and chemogenetics have been developed, which could lead to a giant step toward gene therapy for brain disorders. In this review, we aim to provide an overview of some of the most widely used viral techniques in neuroscience. We will discuss advantages and disadvantages of these methods. In particular, attention is dedicated to the pivotal role played by the introduction of adeno-associated virus and the retrograde tracer canine-associated-2 Cre virus in order to achieve optimal visualization, and interrogation, of chemically-specific neuronal populations and their projections.
AB - Over the last century, there has been great progress in understanding how the brain works. In particular, the last two decades have been crucial in gaining more awareness over the complex functioning of neurotransmitter systems. The use of viral vectors in neuroscience has been pivotal for such development. Exploiting the properties of viral particles, modifying them according to the research needs, and making them target chemically-specific neurons, techniques such as optogenetics and chemogenetics have been developed, which could lead to a giant step toward gene therapy for brain disorders. In this review, we aim to provide an overview of some of the most widely used viral techniques in neuroscience. We will discuss advantages and disadvantages of these methods. In particular, attention is dedicated to the pivotal role played by the introduction of adeno-associated virus and the retrograde tracer canine-associated-2 Cre virus in order to achieve optimal visualization, and interrogation, of chemically-specific neuronal populations and their projections.
KW - AAVs
KW - CAV-2 Cre
KW - Retrograde tracing
KW - Viral tracing
KW - Viral vectors
UR - http://www.scopus.com/inward/record.url?scp=85097065586&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.jneumeth.2020.109004
DO - https://doi.org/10.1016/j.jneumeth.2020.109004
M3 - Article
C2 - 33242528
SN - 0165-0270
VL - 348
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
M1 - 109004
ER -