TY - JOUR
T1 - Transcription regulation of the alpha-glucanase gene agn1 by cell separation transcription factor Ace2p in fission yeast
AU - Dekker, Nick
AU - de Haan, Annett
AU - Hochstenbach, Frans
PY - 2006
Y1 - 2006
N2 - During the final stage of the cell division cycle in the fission yeast Schizosaccharomyces pombe, transcription factor Ace2p activates expression of genes involved in the separation of newly formed daughter cells, such as agn1+, which encodes the alpha-glucanase Agn1p. The agn1 promoter contains three copies of the nucleotide sequence motif CCAGCC, whose presence seems to correlate with Ace2p-mediated transcription activation. Here, we describe a simple plate-based assay utilizing as a reporter the secreted glucoamylase of Arxula adeninivorans to investigate the function of this motif. We show that not all three repeats, but only the two most proximal to the transcription start point, act as an upstream activating sequence (UAS). Finally, we demonstrate that this UAS is essential for agn1 promoter activity in vivo
AB - During the final stage of the cell division cycle in the fission yeast Schizosaccharomyces pombe, transcription factor Ace2p activates expression of genes involved in the separation of newly formed daughter cells, such as agn1+, which encodes the alpha-glucanase Agn1p. The agn1 promoter contains three copies of the nucleotide sequence motif CCAGCC, whose presence seems to correlate with Ace2p-mediated transcription activation. Here, we describe a simple plate-based assay utilizing as a reporter the secreted glucoamylase of Arxula adeninivorans to investigate the function of this motif. We show that not all three repeats, but only the two most proximal to the transcription start point, act as an upstream activating sequence (UAS). Finally, we demonstrate that this UAS is essential for agn1 promoter activity in vivo
U2 - https://doi.org/10.1016/j.febslet.2006.04.061
DO - https://doi.org/10.1016/j.febslet.2006.04.061
M3 - Article
C2 - 16678171
SN - 0014-5793
VL - 580
SP - 3099
EP - 3106
JO - FEBS letters
JF - FEBS letters
IS - 13
ER -