TY - JOUR
T1 - Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity
AU - Roig Adam, Amparo
AU - Martínez-López, José A.
AU - van der Spek, Sophie J. F.
AU - Achsel, Tilmann
AU - Andres-Alonso, Maria
AU - Bagni, Claudia
AU - Bayés, Àlex
AU - Biederer, Thomas
AU - Brose, Nils
AU - Chua, John Jia En
AU - Coba, Marcelo P.
AU - Cornelisse, L. Niels
AU - de Juan-Sanz, Jaime
AU - Goldschmidt, Hana L.
AU - Gundelfinger, Eckart D.
AU - Huganir, Richard L.
AU - Imig, Cordelia
AU - Jahn, Reinhard
AU - Jung, Hwajin
AU - Kaeser, Pascal S.
AU - Kim, Eunjoon
AU - Koopmans, Frank
AU - Kreutz, Michael R.
AU - Lipstein, Noa
AU - MacGillavry, Harold D.
AU - McPherson, Peter S.
AU - O’Connor, Vincent
AU - Pielot, Rainer
AU - Ryan, Timothy A.
AU - Sala, Carlo
AU - Sheng, Morgan
AU - Smalla, Karl-Heinz
AU - The SYNGO consortium
AU - Thomas, Paul D.
AU - Toonen, Ruud F.
AU - van Weering, Jan R. T.
AU - Verpelli, Chiara
AU - Sullivan, Patrick F.
AU - Smit, August B.
AU - Verhage, Matthijs
AU - Hjerling-Leffler, Jens
N1 - Funding Information: Open access funding provided by Karolinska Institute. J.H.-L. was funded by the Swedish Research Council (Vetenskapsrådet, award 2018-00799), European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 819540), and the Swedish Brain Foundation (Hjärnfonden). M.V., A.B.S. and A.R.A. were funded by the Broad Synapse 3 project (6910259-5500000759) and the Simons foundation SFARI director's grant (882976). Publisher Copyright: © 2023, The Author(s).
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Synapse diversity has been described from different perspectives, ranging from the specific neurotransmitters released, to their diverse biophysical properties and proteome profiles. However, synapse diversity at the transcriptional level has not been systematically identified across all synapse populations in the brain. To quantify and identify specific synaptic features of neuronal cell types we combined the SynGO (Synaptic Gene Ontology) database with single-cell RNA sequencing data of the mouse neocortex. We show that cell types can be discriminated by synaptic genes alone with the same power as all genes. The cell type discriminatory power is not equally distributed across synaptic genes as we could identify functional categories and synaptic compartments with greater cell type specific expression. Synaptic genes, and specific SynGO categories, belonged to three different types of gene modules: gradient expression over all cell types, gradient expression in selected cell types and cell class- or type-specific profiles. This data provides a deeper understanding of synapse diversity in the neocortex and identifies potential markers to selectively identify synapses from specific neuronal populations.
AB - Synapse diversity has been described from different perspectives, ranging from the specific neurotransmitters released, to their diverse biophysical properties and proteome profiles. However, synapse diversity at the transcriptional level has not been systematically identified across all synapse populations in the brain. To quantify and identify specific synaptic features of neuronal cell types we combined the SynGO (Synaptic Gene Ontology) database with single-cell RNA sequencing data of the mouse neocortex. We show that cell types can be discriminated by synaptic genes alone with the same power as all genes. The cell type discriminatory power is not equally distributed across synaptic genes as we could identify functional categories and synaptic compartments with greater cell type specific expression. Synaptic genes, and specific SynGO categories, belonged to three different types of gene modules: gradient expression over all cell types, gradient expression in selected cell types and cell class- or type-specific profiles. This data provides a deeper understanding of synapse diversity in the neocortex and identifies potential markers to selectively identify synapses from specific neuronal populations.
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U2 - https://doi.org/10.1186/s13062-023-00372-y
DO - https://doi.org/10.1186/s13062-023-00372-y
M3 - Article
C2 - 37161421
SN - 1745-6150
VL - 18
JO - Biology Direct
JF - Biology Direct
IS - 1
M1 - 22
ER -