Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

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Abstract

Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts
Original languageEnglish
Pages (from-to)63-67
JournalMethods in molecular biology (Clifton, N.J.)
Volume1595
DOIs
Publication statusPublished - 2017

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