Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

Janet Koster; Hans R. Waterham

doi:https://doi.org/10.1007/978-1-4939-6937-1_7

Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

Janet Koster, Hans R. Waterham

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts

Original language	English
Pages (from-to)	63-67
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1595
DOIs	https://doi.org/10.1007/978-1-4939-6937-1_7
Publication status	Published - 2017

Access to Document

https://doi.org/10.1007/978-1-4939-6937-1_7

Cite this

@article{23320780fd0b458f97b4b09521b67b29,

title = "Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies",

abstract = "Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts",

author = "Janet Koster and Waterham, {Hans R.}",

year = "2017",

doi = "https://doi.org/10.1007/978-1-4939-6937-1_7",

language = "English",

volume = "1595",

pages = "63--67",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

AU - Koster, Janet

AU - Waterham, Hans R.

PY - 2017

Y1 - 2017

N2 - Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts

AB - Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts

U2 - https://doi.org/10.1007/978-1-4939-6937-1_7

DO - https://doi.org/10.1007/978-1-4939-6937-1_7

M3 - Article

C2 - 28409452

SN - 1064-3745

VL - 1595

SP - 63

EP - 67

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -