Transgenic Xenopus Iaevis tadpoles: A transient in vivo model system for the manipulation of lens function and lens development

Ruud H. Brakenhoff, Robin C. Ruuls, Eldine H.M. Jacobs, John G.G. Schoenmakers, Nicoltte H. Lubsen

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Rodent γ-crystallin promoters were recognized as lensspecific promoters in micro-injected Xenopus Iaevis tadpoles and targeted the expression of the chloramphenicol acetyl transferase (CAT) reporter gene to the tadpole lens. The onset of expression coincided with lens cell formation. The level of expression continued to increase up to 9 days of development (stage 47), stayed at that level till at least day 13 and dropped by only 57% at day 21. In contrast, the level of expression of a non-tissue-specific promoter, the SV4O early promoter, decreased rapidly in the eye during development and was only detectable up to stage 44 (day 5). The stability of the CAT activity in the lens was assessed by delivering a pulse of activity from a heat shock promoter-CAT fusion gene. The half-life of the CAT activity in the eye was the same as that in the tail. The increase In CAT activity in the lens thus depends upon continued activity of the injected γ crystallin promoters. Our data demonstrate that mammalian promoters can be used to target gene expression to specific tissues during Xenopus laevis development.

Original languageEnglish
Pages (from-to)1279-1284
Number of pages6
JournalNucleic Acids Research
Volume19
Issue number6
DOIs
Publication statusPublished - 25 Mar 1991

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