Abstract
Resident memory T cells (TRM) reside in the lung epithelium and mediate protective immunity against respiratory pathogens. Although lung CD8(+) TRM have been extensively characterized, the properties of CD4(+) TRM remain unclear. Here we determined the transcriptional signature of CD4(+) TRM, identified by the expression of CD103, retrieved from human lung resection material. Various tissue homing molecules were specifically upregulated on CD4(+) TRM, whereas expression of tissue egress and lymph node homing molecules were low. CD103(+) TRM expressed low levels of T-bet, only a small portion expressed Eomesodermin (Eomes), and although the mRNA levels for Hobit were increased, protein expression was absent. On the other hand, the CD103(+) TRM showed a Notch signature. CD4(+)CD103(+) TRM constitutively expressed high transcript levels of numerous cytotoxic mediators that was functionally reflected by a fast recall response, magnitude of cytokine production, and a high degree of polyfunctionality. Interestingly, the superior cytokine production appears to be because of an accessible interferon-γ (IFNγ) locus and was partially because of rapid translation of preformed mRNA. Our studies provide a molecular understanding of the maintenance and potential function of CD4(+) TRM in the human lung. Understanding the specific properties of CD4(+) TRM is required to rationally improve vaccine design.Mucosal Immunology advance online publication 15 November 2017; doi:10.1038/mi.2017.94
Original language | English |
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Pages (from-to) | 654-667 |
Journal | Mucosal Immunology |
Volume | 11 |
Early online date | 2017 |
DOIs | |
Publication status | Published - 2018 |