TY - JOUR
T1 - Type VII collagen in the human accommodation system: Expression in ciliary body, zonules, and lens capsule
AU - Wullink, Bart
AU - Pas, Hendri H.
AU - van der Worp, Roelofje J.
AU - Schol, Martin
AU - Janssen, Sarah F.
AU - Kuijer, Roel
AU - Los, Leonoor I.
PY - 2018
Y1 - 2018
N2 - PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system. METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy. RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized. CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.
AB - PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system. METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy. RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized. CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042528687&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/29490344
U2 - https://doi.org/10.1167/iovs.17-23425
DO - https://doi.org/10.1167/iovs.17-23425
M3 - Article
C2 - 29490344
SN - 0146-0404
VL - 59
SP - 1075
EP - 1083
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 2
ER -