Projects per year
Over the years my research activities have been centered around development and clinical implementation of optical methods for minimally invasive diagnosis and treatment of disease. The work comprises the entire track from basic research and development of methodology to clinical validation and implementation.
The optical diagnostic techniques I have been using have changed over the years. Initially work was focussed around imaging and spectroscopy of autofluorescence and fluorescent contrast agents. In later years the emphasis moved to diffuse reflection spectroscopy and nonlinear optical spectroscopy and imaging. My current research focus lies entirely on elastic scattering spectroscopy and imaging.
Elastic Scattering Spectroscopy uses broadband white light and guides it to the tissue through optical fibers. Light entering the tissue will be scattered around before it is being absorbed, or leaves the tissue at the surface. This backscattered light is detected with an optical fiber and then analysed by a spectrograph. Current research activities have been focussed at development of quantitative approaches, i.e. methods to measure the scattering and absorption spectra quantitatively. So far I developed methods to measure absorption quantitatively, translate these into biologically related parameters and evaluated these extensively in clinical studies. Current focus is on developing methods for quantitative scattering information. Here both the absolute amount of scattering is of interest, but also the relation between forward and backward scattering is of particular interest because it is thought to be sensitive to intracellular biology. Light scattering occurs due to spatial variations in refractive index. Based on analysis of the wavelength dependence of scattering observed in measurements of tissue it can be concluded that the size of these scattering entities lies well below the resolution of an optical microscope (i.e. 100nm). Other types of microscopes can visualize much smaller structures, but do not do so on the basis of the refractive index. Hence the nature of these scattering entities is not known, but it is speculated that they relate to aspects of the cellular microarchitecture, such as folds in mitochondrial membranes, the presence and distribution of endosomes etc. At present I am focussing on measurement forward-to-backward ratio of scattering in vivo. This uses different fiber diameters and will shortly be tested clinically for screening of Barrett’s esophagus, prostate cancer and for diagnosing mediastinal lymph nodes. In addition, a setup is being developed to measure the angular dependence of back scattering on ex vivo samples.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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Sterenborg H.J.C.M.: [NO DATA]
Post, A. L., Swaan, A., Zhang, X., Sterenborg, D. J. C. M. & Kho, E.
12/03/2015 → …
Estimating the risk of squamous cell cancer induction in skin following nonlinear optical imagingThomas, G., Nadiarnykh, O., van Voskuilen, J., Hoy, C. L., Gerritsen, H. C. & Sterenborg, H. J. C. M., 2014, In: Journal of Biophotonics. 7, 7, p. 492-505
Research output: Contribution to journal › Article › Academic › peer-review7 Citations (Scopus)
Validation of Interventional Fiber Optic Spectroscopy With MR Spectroscopy, MAS-NMR Spectroscopy, High-Performance Thin-Layer Chromatography, and Histopathology for Accurate Hepatic Fat QuantificationNachabé, R., van der Hoorn, J. W. A., van de Molengraaf, R., Lamerichs, R., Pikkemaat, J., Sio, C. F., Hendriks, B. H. W. & Sterenborg, H. J. C. M., 2012, In: Investigative Radiology. 47, 4, p. 209-216
Research output: Contribution to journal › Article › Academic › peer-review17 Citations (Scopus)
Differential Pathlength Spectroscopy for the Quantitation of Optical Properties of Gold NanoparticlesUngureanu, C., Amelink, A., Rayavarapu, R. G., Sterenborg, H. J. C. M., Manohar, S. & van Leeuwen, T. G., 2010, In: ACS nano. 4, 7, p. 4081-4089
Research output: Contribution to journal › Article › Academic › peer-review26 Citations (Scopus)
The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopyDoornbos, R. M., Lang, R., Aalders, M. C., Cross, F. W. & Sterenborg, H. J., 1999, In: Physics in Medicine and Biology. 44, 4, p. 967-981
Research output: Contribution to journal › Article › Academic › peer-review368 Citations (Scopus)
Refractive index measurement using single fiber reflectance spectroscopyZhang, X. U., Faber, D. J., Post, A. L., van Leeuwen, T. G. & Sterenborg, H. J. C. M., 2019, In: Journal of Biophotonics. p. e201900019 e201900019.
Research output: Contribution to journal › Article › Academic › peer-reviewOpen Access15 Citations (Scopus)