TY - JOUR
T1 - 1H-N.m.r. study of enzymically generated wheat-endosperm arabinoxylan oligosaccharides: structures of hepta- to tetradeca-saccharides containing two or three branched xylose residues
AU - Hoffmann, R. A.
AU - Geijtenbeek, T.
AU - Kamerling, J. P.
AU - Vliegenthart, J. F.
PY - 1992
Y1 - 1992
N2 - The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1----4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- and/or ----4) [alpha-L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xyl p-(1----, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established
AB - The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1----4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- and/or ----4) [alpha-L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xyl p-(1----, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established
U2 - https://doi.org/10.1016/0008-6215(92)80003-J
DO - https://doi.org/10.1016/0008-6215(92)80003-J
M3 - Article
C2 - 1596918
SN - 0008-6215
VL - 223
SP - 19
EP - 44
JO - Carbohydrate Research
JF - Carbohydrate Research
ER -