TY - JOUR
T1 - Mammalian life depends on two distinct pathways of DNA damage tolerance
AU - Buoninfante, Olimpia Alessandra
AU - Pilzecker, Bas
AU - Spanjaard, Aldo
AU - de Groot, Dani?l
AU - Prekovic, Stefan
AU - Song, Ji-Ying
AU - Lieftink, Cor
AU - Ayidah, Matilda
AU - Pritchard, Colin E. J.
AU - Vivi?, Judith
AU - McGrath, Kathleen E.
AU - Huijbers, Ivo J.
AU - Philipsen, Sjaak
AU - von Lindern, Marieke
AU - Zwart, Wilbert
AU - Beijersbergen, Roderick L.
AU - Palis, James
AU - van den Berk, Paul C. M.
AU - Jacobs, Heinz
N1 - Funding Information: We like to thank N. Wit and F. Alemdehy for comments on the manuscript, members of the FACS facility for assistance during sorting and flow cytometric analysis, the biotechnical staff of the Netherlands Cancer Institute - Antoni van Leeuwenhoek Mouse Clinic for Cancer and Aging (MCCA) for biotechnical support, C. Göbel for help with ChIP-Seq experiments, and all the members of mouse facility for assistance in maintenance of mice. Portions of this work were developed from the doctoral dissertation of O.A.B. Publisher Copyright: Copyright © 2023 the Author(s).
PY - 2023/1/24
Y1 - 2023/1/24
N2 - DNA damage threatens genomic integrity and instigates stem cell failure. To bypass genotoxic lesions during replication, cells employ DNA damage tolerance (DDT), which is regulated via PCNA ubiquitination and REV1. DDT is conserved in all domains of life, yet its relevance in mammals remains unclear. Here, we show that inactivation of both PCNA-ubiquitination and REV1 results in embryonic and adult lethality, and the accumulation of DNA damage in hematopoietic stem and progenitor cells (HSPCs) that ultimately resulted in their depletion. Our results reveal the crucial relevance of DDT in the maintenance of stem cell compartments and mammalian life in unperturbed conditions.
AB - DNA damage threatens genomic integrity and instigates stem cell failure. To bypass genotoxic lesions during replication, cells employ DNA damage tolerance (DDT), which is regulated via PCNA ubiquitination and REV1. DDT is conserved in all domains of life, yet its relevance in mammals remains unclear. Here, we show that inactivation of both PCNA-ubiquitination and REV1 results in embryonic and adult lethality, and the accumulation of DNA damage in hematopoietic stem and progenitor cells (HSPCs) that ultimately resulted in their depletion. Our results reveal the crucial relevance of DDT in the maintenance of stem cell compartments and mammalian life in unperturbed conditions.
KW - DNA damage response (DDR)
KW - DNA damage tolerance (DDT)
KW - embryonic lethality
KW - erythropoiesis
KW - hematopoietic stem cells
UR - http://www.scopus.com/inward/record.url?scp=85146863497&partnerID=8YFLogxK
U2 - https://doi.org/10.1073/pnas.2216055120
DO - https://doi.org/10.1073/pnas.2216055120
M3 - Article
C2 - 36669105
SN - 0027-8424
VL - 120
JO - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
IS - 4
M1 - e2216055120
ER -