A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

J. Gu; E. Märker-Hermann; D. Baeten; W. C. Tsai; D. Gladman; M. Xiong; H. Deister; J. G. Kuipers; F. Huang; Y. W. Song; W. Maksymowych; J. Kalsi; M. Bannai; N. Seta; M. Rihl; L. J. Crofford; E. Veys; F. de Keyser; D. T. Y. Yu

doi:https://doi.org/10.1093/rheumatology/41.7.759

A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

J. Gu, E. Märker-Hermann, D. Baeten, W. C. Tsai, D. Gladman, M. Xiong, H. Deister, J. G. Kuipers, F. Huang, Y. W. Song, W. Maksymowych, J. Kalsi, M. Bannai, N. Seta, M. Rihl, L. J. Crofford, E. Veys, F. de Keyser, D. T. Y. Yu

Clinical Immunology and Rheumatology (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

84 Citations (Scopus)

Abstract

OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA

Original language	English
Pages (from-to)	759-766
Journal	Rheumatology (Oxford, England)
Volume	41
Issue number	7
DOIs	https://doi.org/10.1093/rheumatology/41.7.759
Publication status	Published - 2002

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https://doi.org/10.1093/rheumatology/41.7.759

Cite this

Gu, J., Märker-Hermann, E., Baeten, D., Tsai, W. C., Gladman, D., Xiong, M., Deister, H., Kuipers, J. G., Huang, F., Song, Y. W., Maksymowych, W., Kalsi, J., Bannai, M., Seta, N., Rihl, M., Crofford, L. J., Veys, E., de Keyser, F., & Yu, D. T. Y. (2002). A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients. Rheumatology (Oxford, England), 41(7), 759-766. https://doi.org/10.1093/rheumatology/41.7.759

@article{a3554c4076894985b35ac8c479ef65ac,

title = "A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients",

abstract = "OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA",

author = "J. Gu and E. M{\"a}rker-Hermann and D. Baeten and Tsai, {W. C.} and D. Gladman and M. Xiong and H. Deister and Kuipers, {J. G.} and F. Huang and Song, {Y. W.} and W. Maksymowych and J. Kalsi and M. Bannai and N. Seta and M. Rihl and Crofford, {L. J.} and E. Veys and {de Keyser}, F. and Yu, {D. T. Y.}",

year = "2002",

doi = "https://doi.org/10.1093/rheumatology/41.7.759",

language = "English",

volume = "41",

pages = "759--766",

journal = "Rheumatology (Oxford, England)",

issn = "1462-0324",

publisher = "Oxford University Press",

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Gu, J, Märker-Hermann, E, Baeten, D, Tsai, WC, Gladman, D, Xiong, M, Deister, H, Kuipers, JG, Huang, F, Song, YW, Maksymowych, W, Kalsi, J, Bannai, M, Seta, N, Rihl, M, Crofford, LJ, Veys, E, de Keyser, F & Yu, DTY 2002, 'A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients', Rheumatology (Oxford, England), vol. 41, no. 7, pp. 759-766. https://doi.org/10.1093/rheumatology/41.7.759

TY - JOUR

T1 - A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

AU - Gu, J.

AU - Märker-Hermann, E.

AU - Baeten, D.

AU - Tsai, W. C.

AU - Gladman, D.

AU - Xiong, M.

AU - Deister, H.

AU - Kuipers, J. G.

AU - Huang, F.

AU - Song, Y. W.

AU - Maksymowych, W.

AU - Kalsi, J.

AU - Bannai, M.

AU - Seta, N.

AU - Rihl, M.

AU - Crofford, L. J.

AU - Veys, E.

AU - de Keyser, F.

AU - Yu, D. T. Y.

PY - 2002

Y1 - 2002

N2 - OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA

AB - OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA

U2 - https://doi.org/10.1093/rheumatology/41.7.759

DO - https://doi.org/10.1093/rheumatology/41.7.759

M3 - Article

C2 - 12096225

SN - 1462-0324

VL - 41

SP - 759

EP - 766

JO - Rheumatology (Oxford, England)

JF - Rheumatology (Oxford, England)

IS - 7

ER -