Abstract
Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.
Original language | English |
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Pages (from-to) | 1191-1197 |
Number of pages | 7 |
Journal | Future microbiology |
Volume | 14 |
Issue number | 14 |
DOIs | |
Publication status | Published - 1 Jan 2019 |
Keywords
- aeromonas
- carbapenemase
- long-read sequencing
- minion
- plasmid transfer