TY - JOUR
T1 - A CD34+ human cell line model of myeloid dendritic cell differentiation
T2 - Evidence for a CD14+CD11b+ Langerhans cell precursor
AU - Santegoets, Saskia J.A.M.
AU - Masterson, Allan J.
AU - Van Der Sluis, Pieter C.
AU - Lougheed, Sinéad M.
AU - Fluitsma, Donna M.
AU - Van Den Eertwegh, Alfons J.M.
AU - Pinedo, Herbert M.
AU - Scheper, Rik J.
AU - De Gruijl, Tanja D.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The study of early events in dendritic cell (DC) differentiation is hampered by the lack of homogeneous primary cell systems that allow the study of cytokine-driven, transitional DC differentiation steps. The CD34+ acute myeloid leukemia cell line MUTZ-3 displays a unique ability to differentiate into interstitial DC (IDC) and Langerhans cells (LC) in a cytokine-dependent manner. Phenotypic characterization revealed MUTZ-3 to consist of three distinct subpopulations. Small CD34+CD14 -CD11b- progenitors constitute the proliferative compartment of the cell line with the ability to differentiate through a CD34-CD14- CD11b+ stage to ultimately give rise to a morphologically large, nonproliferating CD14+CD11bhi progeny. These CD14+CD11bhi cells were identified as common, immediate myeloid DC precursors with the ability to differentiate into LC and IDC, exhibiting characteristic and mutually exclusive expression of Langerin and DC-specific ICAM-grabbing nonintegrin, respectively. The identity of the MUTZ-3-derived LC subset was confirmed further by the presence of Birbeck granules. We conclude that the MUTZ-3 cell line provides a ready and continuous supply of common myeloid precursors, which should facilitate further study of the ontogeny of myeloid DC lineages.
AB - The study of early events in dendritic cell (DC) differentiation is hampered by the lack of homogeneous primary cell systems that allow the study of cytokine-driven, transitional DC differentiation steps. The CD34+ acute myeloid leukemia cell line MUTZ-3 displays a unique ability to differentiate into interstitial DC (IDC) and Langerhans cells (LC) in a cytokine-dependent manner. Phenotypic characterization revealed MUTZ-3 to consist of three distinct subpopulations. Small CD34+CD14 -CD11b- progenitors constitute the proliferative compartment of the cell line with the ability to differentiate through a CD34-CD14- CD11b+ stage to ultimately give rise to a morphologically large, nonproliferating CD14+CD11bhi progeny. These CD14+CD11bhi cells were identified as common, immediate myeloid DC precursors with the ability to differentiate into LC and IDC, exhibiting characteristic and mutually exclusive expression of Langerin and DC-specific ICAM-grabbing nonintegrin, respectively. The identity of the MUTZ-3-derived LC subset was confirmed further by the presence of Birbeck granules. We conclude that the MUTZ-3 cell line provides a ready and continuous supply of common myeloid precursors, which should facilitate further study of the ontogeny of myeloid DC lineages.
KW - Acute myeloid leukemia
KW - Cytokines
KW - MUTZ-3
KW - Progenitor
UR - http://www.scopus.com/inward/record.url?scp=33845387051&partnerID=8YFLogxK
U2 - https://doi.org/10.1189/jlb.0206111
DO - https://doi.org/10.1189/jlb.0206111
M3 - Article
C2 - 16959899
SN - 0741-5400
VL - 80
SP - 1337
EP - 1344
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 6
ER -