A comparison of the effects of hyperbaric oxygen culture on survival of murine and canine thyroid gland grafts

T. M. van Gulik; K. Boudjema; K. Platz; D. A. Hullett; J. R. Starling; J. H. Southard; H. W. Sollinger; F. O. Belzer

doi:https://doi.org/10.1007/BF00336408

A comparison of the effects of hyperbaric oxygen culture on survival of murine and canine thyroid gland grafts

T. M. van Gulik, K. Boudjema, K. Platz, D. A. Hullett, J. R. Starling, J. H. Southard, H. W. Sollinger, F. O. Belzer

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by ¹²⁵I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O₂, 5% CO₂; 1.76kg/cm²) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5°C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined: catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.

Original language	English
Pages (from-to)	110-115
Number of pages	6
Journal	Transplant international
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/BF00336408
Publication status	Published - Jun 1991

Keywords

Culture, hyperbaric oxygen
Free radicals, culture, thyroid gland graft
Hypertonic oxygen, thyroid gland graft
Thyroid gland graft, culture

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https://doi.org/10.1007/BF00336408

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title = "A comparison of the effects of hyperbaric oxygen culture on survival of murine and canine thyroid gland grafts",

abstract = "Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by 125I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O2, 5% CO2; 1.76kg/cm2) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5°C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined: catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.",

keywords = "Culture, hyperbaric oxygen, Free radicals, culture, thyroid gland graft, Hypertonic oxygen, thyroid gland graft, Thyroid gland graft, culture",

author = "{van Gulik}, {T. M.} and K. Boudjema and K. Platz and Hullett, {D. A.} and Starling, {J. R.} and Southard, {J. H.} and Sollinger, {H. W.} and Belzer, {F. O.}",

year = "1991",

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doi = "https://doi.org/10.1007/BF00336408",

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AU - van Gulik, T. M.

AU - Boudjema, K.

AU - Platz, K.

AU - Hullett, D. A.

AU - Starling, J. R.

AU - Southard, J. H.

AU - Sollinger, H. W.

AU - Belzer, F. O.

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N2 - Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by 125I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O2, 5% CO2; 1.76kg/cm2) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5°C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined: catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.

AB - Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by 125I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O2, 5% CO2; 1.76kg/cm2) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5°C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined: catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.

KW - Culture, hyperbaric oxygen

KW - Free radicals, culture, thyroid gland graft

KW - Hypertonic oxygen, thyroid gland graft

KW - Thyroid gland graft, culture

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SP - 110

EP - 115

JO - Transplant international

JF - Transplant international

IS - 2

ER -

A comparison of the effects of hyperbaric oxygen culture on survival of murine and canine thyroid gland grafts

Abstract

Keywords

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