TY - JOUR
T1 - A comparison of the quality of image acquisition between the incident dark field and sidestream dark field video-microscopes
AU - Gilbert-Kawai, Edward
AU - Coppel, Jonny
AU - Bountziouka, Vassiliki
AU - Ince, Can
AU - Martin, Daniel
AU - AUTHOR GROUP
AU - Ahuja, V.
AU - Aref-Adib, G.
AU - Burnham, R.
AU - Chisholm, A.
AU - Clarke, K.
AU - Coates, D.
AU - Coates, M.
AU - Cook, D.
AU - Cox, M.
AU - Dhillon, S.
AU - Dougall, C.
AU - Doyle, P.
AU - Duncan, P.
AU - Edsell, M.
AU - Edwards, L.
AU - Evans, L.
AU - Gardiner, P.
AU - Grocott, M.
AU - Gunning, P.
AU - Hart, N.
AU - Harrington, J.
AU - Harvey, J.
AU - Holloway, C.
AU - Howard, D.
AU - Hurlbut, D.
AU - Imray, C.
AU - Jonas, M.
AU - van der Kaaij, J.
AU - Khosravi, M.
AU - Kolfschoten, N.
AU - Levett, D.
AU - Luery, H.
AU - Luks, A.
AU - Martin, D.
AU - McMorrow, R.
AU - Meale, P.
AU - Mitchell, K.
AU - Montgomery, H.
AU - Morgan, G.
AU - Morgan, J.
AU - Murray, A.
AU - Mythen, M.
AU - Newman, S.
AU - O'Dwyer, M.
AU - Pate, J.
PY - 2016
Y1 - 2016
N2 - Background: The 'Cytocam' is a third generation video-microscope, which enables real time visualisation of the in vivo microcirculation. Based upon the principle of incident dark field (IDF) illumination, this hand held computer-controlled device was designed to address the technical limitations of its predecessors, orthogonal polarization spectroscopy and sidestream dark field (SDF) imaging. In this manuscript, we aimed to compare the quality of sublingual microcirculatory image acquisition between the IDF and SDF devices. Methods: Using the microcirculatory image quality scoring (MIQS) system, (six categories scored as either 0 = optimal, 1 = acceptable, or 10 = unacceptable), two independent raters compared 30 films acquired using the Cytocam IDF video-microscope, to an equal number obtained with an SDF device. Blinded to the origin of the films, the raters were therefore able to score between 0 and 60 for each film analysed. The scores' distributions between the two techniques were compared. Results: The median MIQS (95 % CI) given to the SDF camera was 7 (1.5-12), as compared to 1 (0.5-1.0) for the IDF device (p <0.0001). Of the six categories assessed by the MIQS, nearly one fifth of the SDF videos were scored as unacceptable for pressure (20 %), content (20 %), and stability (17 %), with focus scoring deficiently 13 % of the time. High agreement between the two raters scoring values was evident, with an intra-class correlation coefficient (ICC) of 0.96 (95 % CI: 0.94, 0.98). Conclusions: These results demonstrate that the quality of sublingual microcirculatory image acquisition is superior in the Cytocam IDF video-microscope, as compared to the SDF video-microscope
AB - Background: The 'Cytocam' is a third generation video-microscope, which enables real time visualisation of the in vivo microcirculation. Based upon the principle of incident dark field (IDF) illumination, this hand held computer-controlled device was designed to address the technical limitations of its predecessors, orthogonal polarization spectroscopy and sidestream dark field (SDF) imaging. In this manuscript, we aimed to compare the quality of sublingual microcirculatory image acquisition between the IDF and SDF devices. Methods: Using the microcirculatory image quality scoring (MIQS) system, (six categories scored as either 0 = optimal, 1 = acceptable, or 10 = unacceptable), two independent raters compared 30 films acquired using the Cytocam IDF video-microscope, to an equal number obtained with an SDF device. Blinded to the origin of the films, the raters were therefore able to score between 0 and 60 for each film analysed. The scores' distributions between the two techniques were compared. Results: The median MIQS (95 % CI) given to the SDF camera was 7 (1.5-12), as compared to 1 (0.5-1.0) for the IDF device (p <0.0001). Of the six categories assessed by the MIQS, nearly one fifth of the SDF videos were scored as unacceptable for pressure (20 %), content (20 %), and stability (17 %), with focus scoring deficiently 13 % of the time. High agreement between the two raters scoring values was evident, with an intra-class correlation coefficient (ICC) of 0.96 (95 % CI: 0.94, 0.98). Conclusions: These results demonstrate that the quality of sublingual microcirculatory image acquisition is superior in the Cytocam IDF video-microscope, as compared to the SDF video-microscope
U2 - https://doi.org/10.1186/s12880-015-0078-8
DO - https://doi.org/10.1186/s12880-015-0078-8
M3 - Article
C2 - 26797680
SN - 1471-2342
VL - 16
SP - 10
JO - BMC medical imaging
JF - BMC medical imaging
IS - 1
ER -