A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2

C. J. Kuhlemeier; A. A. Thomas; A. van der Ende; R. W. van Leen; W. E. Borrias; C. A. van den Hondel; G. A. van Arkel

doi:https://doi.org/10.1016/0147-619X(83)90068-9

A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2

C. J. Kuhlemeier, A. A. Thomas, A. van der Ende, R. W. van Leen, W. E. Borrias, C. A. van den Hondel, G. A. van Arkel

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Abstract

We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmids was used to construct pUC303, a shuttle vector capable of replication in A. nidulans R2 as well as in Escherichia coli K12. It has two markers, streptomycin and chloramphenicol resistance, and three unique restriction sites. Instability of recombinant plasmids was overcome by using a derivative of A. nidulans R2 cured of the indigenous plasmid pUH24. This strain, R2-SPc, can be transformed stably and at high frequency by the plasmids described in this paper. The combination of the cured strain R2-SPc and the new plasmid pUC303 serves as a suitable host-vector system for gene cloning in cyanobacteria

Original language	English
Pages (from-to)	156-163
Journal	Plasmid
Volume	10
Issue number	2
DOIs	https://doi.org/10.1016/0147-619X(83)90068-9
Publication status	Published - 1983

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https://doi.org/10.1016/0147-619X(83)90068-9

Cite this

@article{99e1564809784ede8f448bb88f72aada,

title = "A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2",

abstract = "We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmids was used to construct pUC303, a shuttle vector capable of replication in A. nidulans R2 as well as in Escherichia coli K12. It has two markers, streptomycin and chloramphenicol resistance, and three unique restriction sites. Instability of recombinant plasmids was overcome by using a derivative of A. nidulans R2 cured of the indigenous plasmid pUH24. This strain, R2-SPc, can be transformed stably and at high frequency by the plasmids described in this paper. The combination of the cured strain R2-SPc and the new plasmid pUC303 serves as a suitable host-vector system for gene cloning in cyanobacteria",

author = "Kuhlemeier, {C. J.} and Thomas, {A. A.} and {van der Ende}, A. and {van Leen}, {R. W.} and Borrias, {W. E.} and {van den Hondel}, {C. A.} and {van Arkel}, {G. A.}",

year = "1983",

doi = "https://doi.org/10.1016/0147-619X(83)90068-9",

language = "English",

volume = "10",

pages = "156--163",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2

AU - Kuhlemeier, C. J.

AU - Thomas, A. A.

AU - van der Ende, A.

AU - van Leen, R. W.

AU - Borrias, W. E.

AU - van den Hondel, C. A.

AU - van Arkel, G. A.

PY - 1983

Y1 - 1983

N2 - We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmids was used to construct pUC303, a shuttle vector capable of replication in A. nidulans R2 as well as in Escherichia coli K12. It has two markers, streptomycin and chloramphenicol resistance, and three unique restriction sites. Instability of recombinant plasmids was overcome by using a derivative of A. nidulans R2 cured of the indigenous plasmid pUH24. This strain, R2-SPc, can be transformed stably and at high frequency by the plasmids described in this paper. The combination of the cured strain R2-SPc and the new plasmid pUC303 serves as a suitable host-vector system for gene cloning in cyanobacteria

AB - We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmids was used to construct pUC303, a shuttle vector capable of replication in A. nidulans R2 as well as in Escherichia coli K12. It has two markers, streptomycin and chloramphenicol resistance, and three unique restriction sites. Instability of recombinant plasmids was overcome by using a derivative of A. nidulans R2 cured of the indigenous plasmid pUH24. This strain, R2-SPc, can be transformed stably and at high frequency by the plasmids described in this paper. The combination of the cured strain R2-SPc and the new plasmid pUC303 serves as a suitable host-vector system for gene cloning in cyanobacteria

U2 - https://doi.org/10.1016/0147-619X(83)90068-9

DO - https://doi.org/10.1016/0147-619X(83)90068-9

M3 - Article

C2 - 6314409

SN - 0147-619X

VL - 10

SP - 156

EP - 163

JO - Plasmid

JF - Plasmid

IS - 2

ER -