A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue

David C Hondius; Jeroen J M Hoozemans; Annemieke J M Rozemuller; Ka Wan Li; August B Smit

doi:https://doi.org/10.1007/978-1-4939-7558-7_21

A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue

David C Hondius, Jeroen J M Hoozemans, Annemieke J M Rozemuller, Ka Wan Li, August B Smit

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

Original language	English
Pages (from-to)	371-383
Number of pages	13
Journal	Methods in Molecular Biology
Volume	1723
DOIs	https://doi.org/10.1007/978-1-4939-7558-7_21
Publication status	Published - 2018

Keywords

Journal Article

Access to Document

https://doi.org/10.1007/978-1-4939-7558-7_21

Cite this

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title = "A Laser Microdissection-Liquid Chromatography-Tandem Mass Spectrometry Workflow for Post-mortem Analysis of Brain Tissue",

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AU - Li, Ka Wan

AU - Smit, August B

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AB - Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

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