A liposomal system for contrast-enhanced magnetic resonance imaging of molecular targets

Willem J. M. Mulder; Gustav J. Strijkers; Arjan W. Griffioen; Louis van Bloois; Grietje Molema; Gert Storm; Gerben A. Koning; Klaas Nicolay

doi:https://doi.org/10.1021/bc049949r

A liposomal system for contrast-enhanced magnetic resonance imaging of molecular targets

Willem J. M. Mulder, Gustav J. Strijkers, Arjan W. Griffioen, Louis van Bloois, Grietje Molema, Gert Storm, Gerben A. Koning, Klaas Nicolay

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Pegylated paramagnetic and fluorescent immunoliposomes were designed to enable the parallel detection of the induced expression of molecular markers on endothelial cells with magnetic resonance imaging (MRI) and fluorescence microscopy. MRI is capable of three-dimensional noninvasive imaging of opaque tissues at near cellular resolution, while fluorescence microscopy can be used to investigate processes at the subcellular level. As a model for the expression of a molecular marker, human umbilical vein endothelial cells (HUVEC) were treated with the pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha) to upregulate the expression of the adhesion molecule E-selectin/CD62E. E-selectin-expressing HUVEC were incubated with pegylated paramagnetic fluorescently labeled liposomes carrying anti-E-selectin monoclonal antibody as a targeting ligand. Both MRI and fluorescence microscopy revealed the specific association of the liposomal MR contrast agent with stimulated HUVEC. This study suggests that this newly developed system may serve as a useful diagnostic tool to investigate pathological processes in vivo with MRI

Original language	English
Pages (from-to)	799-806
Journal	Bioconjugate chemistry
Volume	15
Issue number	4
DOIs	https://doi.org/10.1021/bc049949r
Publication status	Published - 2004

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https://doi.org/10.1021/bc049949r

Cite this

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title = "A liposomal system for contrast-enhanced magnetic resonance imaging of molecular targets",

abstract = "Pegylated paramagnetic and fluorescent immunoliposomes were designed to enable the parallel detection of the induced expression of molecular markers on endothelial cells with magnetic resonance imaging (MRI) and fluorescence microscopy. MRI is capable of three-dimensional noninvasive imaging of opaque tissues at near cellular resolution, while fluorescence microscopy can be used to investigate processes at the subcellular level. As a model for the expression of a molecular marker, human umbilical vein endothelial cells (HUVEC) were treated with the pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha) to upregulate the expression of the adhesion molecule E-selectin/CD62E. E-selectin-expressing HUVEC were incubated with pegylated paramagnetic fluorescently labeled liposomes carrying anti-E-selectin monoclonal antibody as a targeting ligand. Both MRI and fluorescence microscopy revealed the specific association of the liposomal MR contrast agent with stimulated HUVEC. This study suggests that this newly developed system may serve as a useful diagnostic tool to investigate pathological processes in vivo with MRI",

author = "Mulder, {Willem J. M.} and Strijkers, {Gustav J.} and Griffioen, {Arjan W.} and {van Bloois}, Louis and Grietje Molema and Gert Storm and Koning, {Gerben A.} and Klaas Nicolay",

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doi = "https://doi.org/10.1021/bc049949r",

language = "English",

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publisher = "American Chemical Society",

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T1 - A liposomal system for contrast-enhanced magnetic resonance imaging of molecular targets

AU - Mulder, Willem J. M.

AU - Strijkers, Gustav J.

AU - Griffioen, Arjan W.

AU - van Bloois, Louis

AU - Molema, Grietje

AU - Storm, Gert

AU - Koning, Gerben A.

AU - Nicolay, Klaas

PY - 2004

Y1 - 2004

N2 - Pegylated paramagnetic and fluorescent immunoliposomes were designed to enable the parallel detection of the induced expression of molecular markers on endothelial cells with magnetic resonance imaging (MRI) and fluorescence microscopy. MRI is capable of three-dimensional noninvasive imaging of opaque tissues at near cellular resolution, while fluorescence microscopy can be used to investigate processes at the subcellular level. As a model for the expression of a molecular marker, human umbilical vein endothelial cells (HUVEC) were treated with the pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha) to upregulate the expression of the adhesion molecule E-selectin/CD62E. E-selectin-expressing HUVEC were incubated with pegylated paramagnetic fluorescently labeled liposomes carrying anti-E-selectin monoclonal antibody as a targeting ligand. Both MRI and fluorescence microscopy revealed the specific association of the liposomal MR contrast agent with stimulated HUVEC. This study suggests that this newly developed system may serve as a useful diagnostic tool to investigate pathological processes in vivo with MRI

AB - Pegylated paramagnetic and fluorescent immunoliposomes were designed to enable the parallel detection of the induced expression of molecular markers on endothelial cells with magnetic resonance imaging (MRI) and fluorescence microscopy. MRI is capable of three-dimensional noninvasive imaging of opaque tissues at near cellular resolution, while fluorescence microscopy can be used to investigate processes at the subcellular level. As a model for the expression of a molecular marker, human umbilical vein endothelial cells (HUVEC) were treated with the pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha) to upregulate the expression of the adhesion molecule E-selectin/CD62E. E-selectin-expressing HUVEC were incubated with pegylated paramagnetic fluorescently labeled liposomes carrying anti-E-selectin monoclonal antibody as a targeting ligand. Both MRI and fluorescence microscopy revealed the specific association of the liposomal MR contrast agent with stimulated HUVEC. This study suggests that this newly developed system may serve as a useful diagnostic tool to investigate pathological processes in vivo with MRI

U2 - https://doi.org/10.1021/bc049949r

DO - https://doi.org/10.1021/bc049949r

M3 - Article

C2 - 15264867

SN - 1043-1802

VL - 15

SP - 799

EP - 806

JO - Bioconjugate chemistry

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