A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa

Floortje Strobbe, M. lanie V. Bénard, Noortje G. Rossen, Willem M. de Vos, Nitin Kumar, Trevor D. Lawley, Erwin G. Zoetendal, Floor Hugenholtz, Cyriel Y. Ponsioen

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Abstract

We evaluated a novel ‘protected’ biopsy method to reliably ascertain the spatial distribution of the mucosa-adherent colonic microbiota. Apart from minor differences at genus level, overall similarities along the colon were high between the various areas, irrespective of protected or unprotected sampling.
Original languageEnglish
Article number106204
JournalJournal of Microbiological Methods
Volume185
DOIs
Publication statusPublished - 1 Jun 2021

Keywords

  • Mucosa-adherent colonic microbiota
  • Protected biopsy technique

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