TY - JOUR
T1 - A prolonged fast improves overnight substrate oxidation without modulating hepatic glycogen in adults with and without nonalcoholic fatty liver
T2 - A randomized crossover trial
AU - Roumans, Kay H. M.
AU - Veelen, Anna
AU - Andriessen, Charlotte
AU - Mevenkamp, Julian
AU - Kornips, Esther
AU - Veeraiah, Pandichelvam
AU - Havekes, Bas
AU - Peters, Harry P. F.
AU - Lindeboom, Lucas
AU - Schrauwen, Patrick
AU - Schrauwen-Hinderling, Vera B.
N1 - Funding Information: The authors acknowledge the support from the Netherlands Cardiovascular Research Initiative: an initiative with support of the Dutch Heart Foundation (CVON2014‐02 ENERGISE). The authors thank all the enthusiastic volunteers in this study for their participation. Data described in the manuscript will be made available upon request, pending approval. Funding Information: This research was in part financed by the Ministry of Economic Affairs and Climate Policy by means of the public–private partnership allowance made available by Top Sector Life Sciences & Health to stimulate public–private partnerships and by Unilever Research and Development, Wageningen. Vera Shrauwen‐Hinderling was supported by a European Research Council starting grant (grant number 759161, “MRS in Diabetes”). Publisher Copyright: © 2023 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society.
PY - 2023/3/1
Y1 - 2023/3/1
N2 - Objective: Increasing overnight fasting time seems a promising strategy to improve metabolic health in individuals with nonalcoholic fatty liver (NAFL). Mechanisms underlying the beneficial effects of fasting may be related to larger fluctuations in hepatic glycogen and higher fat oxidation. This study investigated whether prolonging an overnight fast depletes hepatic glycogen stores and improves substrate metabolism in individuals with NAFL and healthy lean individuals. Methods: Eleven individuals with NAFL and ten control individuals participated in this randomized crossover trial. After a 9.5-hour or 16-hour fast, hepatic glycogen was measured by using carbon-13 magnetic resonance spectroscopy, and a meal test was performed. Nocturnal substrate oxidation was measured with indirect calorimetry. Results: Extending fasting time led to lower nocturnal carbohydrate oxidation and higher fat oxidation in both groups (intervention × time, p < 0.005 for carbohydrate and fat oxidation). In both arms, the respiratory exchange ratio measured during the night remained higher in the group with NAFL compared with the control group (population p < 0.001). No changes were observed in hepatic glycogen depletion with a prolonged overnight fast in the group with NAFL or the control group. Conclusions: These results suggest that acutely prolonging the overnight fast can improve overnight substrate oxidation and that these alterations are not mediated by changes in hepatic glycogen depletion.
AB - Objective: Increasing overnight fasting time seems a promising strategy to improve metabolic health in individuals with nonalcoholic fatty liver (NAFL). Mechanisms underlying the beneficial effects of fasting may be related to larger fluctuations in hepatic glycogen and higher fat oxidation. This study investigated whether prolonging an overnight fast depletes hepatic glycogen stores and improves substrate metabolism in individuals with NAFL and healthy lean individuals. Methods: Eleven individuals with NAFL and ten control individuals participated in this randomized crossover trial. After a 9.5-hour or 16-hour fast, hepatic glycogen was measured by using carbon-13 magnetic resonance spectroscopy, and a meal test was performed. Nocturnal substrate oxidation was measured with indirect calorimetry. Results: Extending fasting time led to lower nocturnal carbohydrate oxidation and higher fat oxidation in both groups (intervention × time, p < 0.005 for carbohydrate and fat oxidation). In both arms, the respiratory exchange ratio measured during the night remained higher in the group with NAFL compared with the control group (population p < 0.001). No changes were observed in hepatic glycogen depletion with a prolonged overnight fast in the group with NAFL or the control group. Conclusions: These results suggest that acutely prolonging the overnight fast can improve overnight substrate oxidation and that these alterations are not mediated by changes in hepatic glycogen depletion.
UR - http://www.scopus.com/inward/record.url?scp=85147597396&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/oby.23676
DO - https://doi.org/10.1002/oby.23676
M3 - Article
C2 - 36756887
SN - 1930-7381
VL - 31
SP - 757
EP - 767
JO - Obesity
JF - Obesity
IS - 3
ER -