A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration

M.C. van den Oever; S. Spijker; K.W. Li; C.R. Jimenez; E. Koya; R.C. van der Schors; Y. Gouwenberg; R. Binnekade; T.J. de Vries; A.N.M. Schoffelmeer; A.B. Smit

doi:https://doi.org/10.1021/pr050303y

A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration

M.C. van den Oever, S. Spijker, K.W. Li, C.R. Jimenez, E. Koya, R.C. van der Schors, Y. Gouwenberg, R. Binnekade, T.J. de Vries, A.N.M. Schoffelmeer, A.B. Smit

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

The medial prefrontal cortex (mPFC) is involved in the processing and retrieval of reward-related information. Here, we investigated long-lasting changes in protein composition of the mPFC in rats with a history of sucrose self-administration. Protein levels were analyzed using 2-D PAGE and MALDITOF sequencing. From ∼1500 spots, 28 regulated proteins were unambiguously identified and were involved in cytoskeleton organization, energy metabolism, oxidative stress, neurotransmission, and neuronal outgrowth and differentiation. For several proteins, this change was also found as a long-lasting alteration in gene expression. We show that self-administration of sucrose produces long-lasting molecular neuroadaptations in the mPFC that may be involved in reward-related information processing. © 2006 American Chemical Society.

Original language	English
Pages (from-to)	147-154
Number of pages	8
Journal	Journal of proteome research
Volume	5
Issue number	1
DOIs	https://doi.org/10.1021/pr050303y
Publication status	Published - Jan 2006

Keywords

Animals
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation
Journal Article
Male
Prefrontal Cortex
Proteins
Proteomics
Rats
Rats, Wistar
Research Support, Non-U.S. Gov't
Self Administration
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sucrose

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1021/pr050303y

Cite this

van den Oever, M. C., Spijker, S., Li, K. W., Jimenez, C. R., Koya, E., van der Schors, R. C., Gouwenberg, Y., Binnekade, R., de Vries, T. J., Schoffelmeer, A. N. M., & Smit, A. B. (2006). A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration. Journal of proteome research, 5(1), 147-154. https://doi.org/10.1021/pr050303y

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title = "A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration",

abstract = "The medial prefrontal cortex (mPFC) is involved in the processing and retrieval of reward-related information. Here, we investigated long-lasting changes in protein composition of the mPFC in rats with a history of sucrose self-administration. Protein levels were analyzed using 2-D PAGE and MALDITOF sequencing. From ∼1500 spots, 28 regulated proteins were unambiguously identified and were involved in cytoskeleton organization, energy metabolism, oxidative stress, neurotransmission, and neuronal outgrowth and differentiation. For several proteins, this change was also found as a long-lasting alteration in gene expression. We show that self-administration of sucrose produces long-lasting molecular neuroadaptations in the mPFC that may be involved in reward-related information processing. {\textcopyright} 2006 American Chemical Society.",

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author = "{van den Oever}, M.C. and S. Spijker and K.W. Li and C.R. Jimenez and E. Koya and {van der Schors}, R.C. and Y. Gouwenberg and R. Binnekade and {de Vries}, T.J. and A.N.M. Schoffelmeer and A.B. Smit",

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van den Oever, MC, Spijker, S, Li, KW, Jimenez, CR, Koya, E, van der Schors, RC, Gouwenberg, Y, Binnekade, R, de Vries, TJ, Schoffelmeer, ANM & Smit, AB 2006, 'A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration', Journal of proteome research, vol. 5, no. 1, pp. 147-154. https://doi.org/10.1021/pr050303y

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AU - van den Oever, M.C.

AU - Spijker, S.

AU - Li, K.W.

AU - Jimenez, C.R.

AU - Koya, E.

AU - van der Schors, R.C.

AU - Gouwenberg, Y.

AU - Binnekade, R.

AU - de Vries, T.J.

AU - Schoffelmeer, A.N.M.

AU - Smit, A.B.

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AB - The medial prefrontal cortex (mPFC) is involved in the processing and retrieval of reward-related information. Here, we investigated long-lasting changes in protein composition of the mPFC in rats with a history of sucrose self-administration. Protein levels were analyzed using 2-D PAGE and MALDITOF sequencing. From ∼1500 spots, 28 regulated proteins were unambiguously identified and were involved in cytoskeleton organization, energy metabolism, oxidative stress, neurotransmission, and neuronal outgrowth and differentiation. For several proteins, this change was also found as a long-lasting alteration in gene expression. We show that self-administration of sucrose produces long-lasting molecular neuroadaptations in the mPFC that may be involved in reward-related information processing. © 2006 American Chemical Society.

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KW - Proteins

KW - Proteomics

KW - Rats

KW - Rats, Wistar

KW - Research Support, Non-U.S. Gov't

KW - Self Administration

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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