A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

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Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.
Original languageEnglish
Pages (from-to)235-251
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2023


  • Clinically applicable
  • Extracellular vesicles
  • Isolation of urinary extracellular vesicles
  • Mass spectrometry
  • Proteomics
  • Urine

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