Abstract
Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.
Original language | English |
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Pages (from-to) | 235-251 |
Number of pages | 17 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 2718 |
DOIs | |
Publication status | Published - 2023 |
Keywords
- Clinically applicable
- Extracellular vesicles
- Isolation of urinary extracellular vesicles
- Mass spectrometry
- Proteomics
- Urine