A sensitive cell-based assay for the detection of residual infectious West Nile virus

M. H. Koldijk; J. A. Bogaards; S. Kostense; M. de Vocht; L. Gijsbers; M. ter Haak; C. Ophorst; J. P.J. Brakenhoff; G. J. Weverling; J. Y. Guichoux; F. UytdeHaag; J. Lewis; J. Goudsmit; G. Marzio

doi:https://doi.org/10.1016/j.vaccine.2007.07.011

A sensitive cell-based assay for the detection of residual infectious West Nile virus

M. H. Koldijk, J. A. Bogaards, S. Kostense, M. de Vocht, L. Gijsbers, M. ter Haak, C. Ophorst, J. P.J. Brakenhoff, G. J. Weverling, J. Y. Guichoux, F. UytdeHaag, J. Lewis, J. Goudsmit, G. Marzio

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

Ensuring complete viral inactivation is critical for the safety of vaccines based on an inactivated virus. Detection of residual infectious virus is dependent on sensitivity of the assay, sample volume analyzed and the absence of interference with viral infection. Here we describe the development and qualification of a sensitive cell-based assay for the detection of residual infectious West Nile Virus (WNV). The results of the assay are in good agreement with the assumption that at low concentrations the number of infectious units in relatively small samples follows a Poisson distribution. The assay can detect 1 infectious unit with a confidence of 99%, provides statistical controls for interference and can easily be scaled up to test large amounts of vaccine material. Furthermore, we show equivalence in sensitivity between the cell-based assay and an in vivo assay for detection of infectious WNV. Finally, the assay has been used for successful release testing of clinical lots of inactivated WNV vaccine. Given the principle and generic setup of the method we envision broad applicability to the detection of very low concentrations of infectious virus.

Original language	English
Pages (from-to)	6872-6881
Number of pages	10
Journal	Vaccine
Volume	25
Issue number	39-40
DOIs	https://doi.org/10.1016/j.vaccine.2007.07.011
Publication status	Published - 28 Sept 2007

Keywords

Assay
Inactivation
Virus

Access to Document

https://doi.org/10.1016/j.vaccine.2007.07.011

Cite this

Koldijk, M. H., Bogaards, J. A., Kostense, S., de Vocht, M., Gijsbers, L., ter Haak, M., Ophorst, C., Brakenhoff, J. P. J., Weverling, G. J., Guichoux, J. Y., UytdeHaag, F., Lewis, J., Goudsmit, J., & Marzio, G. (2007). A sensitive cell-based assay for the detection of residual infectious West Nile virus. Vaccine, 25(39-40), 6872-6881. https://doi.org/10.1016/j.vaccine.2007.07.011

@article{856216993fea40a58f3248b2baf1f4b1,

title = "A sensitive cell-based assay for the detection of residual infectious West Nile virus",

abstract = "Ensuring complete viral inactivation is critical for the safety of vaccines based on an inactivated virus. Detection of residual infectious virus is dependent on sensitivity of the assay, sample volume analyzed and the absence of interference with viral infection. Here we describe the development and qualification of a sensitive cell-based assay for the detection of residual infectious West Nile Virus (WNV). The results of the assay are in good agreement with the assumption that at low concentrations the number of infectious units in relatively small samples follows a Poisson distribution. The assay can detect 1 infectious unit with a confidence of 99%, provides statistical controls for interference and can easily be scaled up to test large amounts of vaccine material. Furthermore, we show equivalence in sensitivity between the cell-based assay and an in vivo assay for detection of infectious WNV. Finally, the assay has been used for successful release testing of clinical lots of inactivated WNV vaccine. Given the principle and generic setup of the method we envision broad applicability to the detection of very low concentrations of infectious virus.",

keywords = "Assay, Inactivation, Virus",

author = "Koldijk, {M. H.} and Bogaards, {J. A.} and S. Kostense and {de Vocht}, M. and L. Gijsbers and {ter Haak}, M. and C. Ophorst and Brakenhoff, {J. P.J.} and Weverling, {G. J.} and Guichoux, {J. Y.} and F. UytdeHaag and J. Lewis and J. Goudsmit and G. Marzio",

year = "2007",

month = sep,

day = "28",

doi = "https://doi.org/10.1016/j.vaccine.2007.07.011",

language = "English",

volume = "25",

pages = "6872--6881",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "39-40",

}

TY - JOUR

T1 - A sensitive cell-based assay for the detection of residual infectious West Nile virus

AU - Koldijk, M. H.

AU - Bogaards, J. A.

AU - Kostense, S.

AU - de Vocht, M.

AU - Gijsbers, L.

AU - ter Haak, M.

AU - Ophorst, C.

AU - Brakenhoff, J. P.J.

AU - Weverling, G. J.

AU - Guichoux, J. Y.

AU - UytdeHaag, F.

AU - Lewis, J.

AU - Goudsmit, J.

AU - Marzio, G.

PY - 2007/9/28

Y1 - 2007/9/28

N2 - Ensuring complete viral inactivation is critical for the safety of vaccines based on an inactivated virus. Detection of residual infectious virus is dependent on sensitivity of the assay, sample volume analyzed and the absence of interference with viral infection. Here we describe the development and qualification of a sensitive cell-based assay for the detection of residual infectious West Nile Virus (WNV). The results of the assay are in good agreement with the assumption that at low concentrations the number of infectious units in relatively small samples follows a Poisson distribution. The assay can detect 1 infectious unit with a confidence of 99%, provides statistical controls for interference and can easily be scaled up to test large amounts of vaccine material. Furthermore, we show equivalence in sensitivity between the cell-based assay and an in vivo assay for detection of infectious WNV. Finally, the assay has been used for successful release testing of clinical lots of inactivated WNV vaccine. Given the principle and generic setup of the method we envision broad applicability to the detection of very low concentrations of infectious virus.

AB - Ensuring complete viral inactivation is critical for the safety of vaccines based on an inactivated virus. Detection of residual infectious virus is dependent on sensitivity of the assay, sample volume analyzed and the absence of interference with viral infection. Here we describe the development and qualification of a sensitive cell-based assay for the detection of residual infectious West Nile Virus (WNV). The results of the assay are in good agreement with the assumption that at low concentrations the number of infectious units in relatively small samples follows a Poisson distribution. The assay can detect 1 infectious unit with a confidence of 99%, provides statistical controls for interference and can easily be scaled up to test large amounts of vaccine material. Furthermore, we show equivalence in sensitivity between the cell-based assay and an in vivo assay for detection of infectious WNV. Finally, the assay has been used for successful release testing of clinical lots of inactivated WNV vaccine. Given the principle and generic setup of the method we envision broad applicability to the detection of very low concentrations of infectious virus.

KW - Assay

KW - Inactivation

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=34548569040&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.vaccine.2007.07.011

DO - https://doi.org/10.1016/j.vaccine.2007.07.011

M3 - Article

C2 - 17707954

SN - 0264-410X

VL - 25

SP - 6872

EP - 6881

JO - Vaccine

JF - Vaccine

IS - 39-40

ER -

A sensitive cell-based assay for the detection of residual infectious West Nile virus

Abstract

Keywords

Access to Document

Other files and links

Cite this