A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans

Arwen W. Gao; Iliana A. Chatzispyrou; Rashmi Kamble; Yasmine J. Liu; Katharina Herzog; Reuben L. Smith; Henk van Lenthe; Martin A. T. Vervaart; Arno van Cruchten; Angela C. Luyf; Antoine van Kampen; Mia L. Pras-Raves; Frédéric M. Vaz; Riekelt H. Houtkooper

doi:https://doi.org/10.1038/s41598-017-02539-w

A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans

Arwen W. Gao, Iliana A. Chatzispyrou, Rashmi Kamble, Yasmine J. Liu, Katharina Herzog, Reuben L. Smith, Henk van Lenthe, Martin A. T. Vervaart, Arno van Cruchten, Angela C. Luyf, Antoine van Kampen, Mia L. Pras-Raves, Frédéric M. Vaz, Riekelt H. Houtkooper

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. Based on recent advances in the metabolomics field, we optimized and validated a sensitive mass spectrometry (MS) platform for identification of major metabolite classes in worms and applied it to study age and diet related changes. Using this platform that allowed detection of over 600 metabolites in a sample of 2500 worms, we observed marked changes in fatty acids, amino acids and phospholipids during worm life history, which were independent from the germ-line. Worms underwent a striking shift in lipid metabolism after early adulthood that was at least partly controlled by the metabolic regulator AAK-2/AMPK. Most amino acids peaked during development, except aspartic acid and glycine, which accumulated in aged worms. Dietary intervention also influenced worm metabolite profiles and the regulation was highly specific depending on the metabolite class. Altogether, these MS-based methods are powerful tools to perform worm metabolomics for aging and metabolism-oriented studies

Original language	English
Pages (from-to)	2408
Journal	Scientific reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-02539-w
Publication status	Published - 2017

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Cite this

Gao, A. W., Chatzispyrou, I. A., Kamble, R., Liu, Y. J., Herzog, K., Smith, R. L., van Lenthe, H., Vervaart, M. A. T., van Cruchten, A., Luyf, A. C., van Kampen, A., Pras-Raves, M. L., Vaz, F. M., & Houtkooper, R. H. (2017). A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans. Scientific reports, 7(1), 2408. https://doi.org/10.1038/s41598-017-02539-w

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title = "A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans",

abstract = "Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. Based on recent advances in the metabolomics field, we optimized and validated a sensitive mass spectrometry (MS) platform for identification of major metabolite classes in worms and applied it to study age and diet related changes. Using this platform that allowed detection of over 600 metabolites in a sample of 2500 worms, we observed marked changes in fatty acids, amino acids and phospholipids during worm life history, which were independent from the germ-line. Worms underwent a striking shift in lipid metabolism after early adulthood that was at least partly controlled by the metabolic regulator AAK-2/AMPK. Most amino acids peaked during development, except aspartic acid and glycine, which accumulated in aged worms. Dietary intervention also influenced worm metabolite profiles and the regulation was highly specific depending on the metabolite class. Altogether, these MS-based methods are powerful tools to perform worm metabolomics for aging and metabolism-oriented studies",

author = "Gao, {Arwen W.} and Chatzispyrou, {Iliana A.} and Rashmi Kamble and Liu, {Yasmine J.} and Katharina Herzog and Smith, {Reuben L.} and {van Lenthe}, Henk and Vervaart, {Martin A. T.} and {van Cruchten}, Arno and Luyf, {Angela C.} and {van Kampen}, Antoine and Pras-Raves, {Mia L.} and Vaz, {Fr{\'e}d{\'e}ric M.} and Houtkooper, {Riekelt H.}",

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Gao, AW, Chatzispyrou, IA, Kamble, R, Liu, YJ, Herzog, K, Smith, RL, van Lenthe, H, Vervaart, MAT, van Cruchten, A, Luyf, AC, van Kampen, A , Pras-Raves, ML , Vaz, FM & Houtkooper, RH 2017, 'A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans', Scientific reports, vol. 7, no. 1, pp. 2408. https://doi.org/10.1038/s41598-017-02539-w

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AU - Gao, Arwen W.

AU - Chatzispyrou, Iliana A.

AU - Kamble, Rashmi

AU - Liu, Yasmine J.

AU - Herzog, Katharina

AU - Smith, Reuben L.

AU - van Lenthe, Henk

AU - Vervaart, Martin A. T.

AU - van Cruchten, Arno

AU - Luyf, Angela C.

AU - van Kampen, Antoine

AU - Pras-Raves, Mia L.

AU - Vaz, Frédéric M.

AU - Houtkooper, Riekelt H.

PY - 2017

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AB - Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. Based on recent advances in the metabolomics field, we optimized and validated a sensitive mass spectrometry (MS) platform for identification of major metabolite classes in worms and applied it to study age and diet related changes. Using this platform that allowed detection of over 600 metabolites in a sample of 2500 worms, we observed marked changes in fatty acids, amino acids and phospholipids during worm life history, which were independent from the germ-line. Worms underwent a striking shift in lipid metabolism after early adulthood that was at least partly controlled by the metabolic regulator AAK-2/AMPK. Most amino acids peaked during development, except aspartic acid and glycine, which accumulated in aged worms. Dietary intervention also influenced worm metabolite profiles and the regulation was highly specific depending on the metabolite class. Altogether, these MS-based methods are powerful tools to perform worm metabolomics for aging and metabolism-oriented studies

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DO - https://doi.org/10.1038/s41598-017-02539-w

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