Accuracy of erythropoietin determination in the dialysate of CAPD patients

D. G. Struijk; G. C. Koomen; R. T. Krediet; L. Arisz

Accuracy of erythropoietin determination in the dialysate of CAPD patients

D. G. Struijk, G. C. Koomen, R. T. Krediet, L. Arisz

Other Departments

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

In vitro experiments were performed to analyze problems with the determination of erythropoietin in dialysate. Human recombinant erythropoietin (EPO; 4000 U/L) was added to several fluids, to glass or polystyrene tubes with or without addition of bovine serum albumin (BSA) and to dialysate bags. The recovery in peritoneal effluent was 4120 +/- 203 U/L (mean +/- SEM). The recovery in the other fluids was less than 50% but in glass tubes it increased to 96% after addition of BSA. Binding was also found to the dialysate bag, therefore reducing the amount available for absorption. We recommend that EPO samples from the dialysate are drawn within BSA coated glass tubes

Original language	English
Pages (from-to)	289-291
Journal	Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
Volume	6
Publication status	Published - 1990

Cite this

@article{a057cbcc70d04660b4f3b640443d2feb,

title = "Accuracy of erythropoietin determination in the dialysate of CAPD patients",

abstract = "In vitro experiments were performed to analyze problems with the determination of erythropoietin in dialysate. Human recombinant erythropoietin (EPO; 4000 U/L) was added to several fluids, to glass or polystyrene tubes with or without addition of bovine serum albumin (BSA) and to dialysate bags. The recovery in peritoneal effluent was 4120 +/- 203 U/L (mean +/- SEM). The recovery in the other fluids was less than 50% but in glass tubes it increased to 96% after addition of BSA. Binding was also found to the dialysate bag, therefore reducing the amount available for absorption. We recommend that EPO samples from the dialysate are drawn within BSA coated glass tubes",

author = "Struijk, {D. G.} and Koomen, {G. C.} and Krediet, {R. T.} and L. Arisz",

year = "1990",

language = "English",

volume = "6",

pages = "289--291",

journal = "Advances in peritoneal dialysis. Conference on Peritoneal Dialysis",

issn = "1197-8554",

publisher = "Peritoneal Dialysis Bulletin",

}

TY - JOUR

T1 - Accuracy of erythropoietin determination in the dialysate of CAPD patients

AU - Struijk, D. G.

AU - Koomen, G. C.

AU - Krediet, R. T.

AU - Arisz, L.

PY - 1990

Y1 - 1990

N2 - In vitro experiments were performed to analyze problems with the determination of erythropoietin in dialysate. Human recombinant erythropoietin (EPO; 4000 U/L) was added to several fluids, to glass or polystyrene tubes with or without addition of bovine serum albumin (BSA) and to dialysate bags. The recovery in peritoneal effluent was 4120 +/- 203 U/L (mean +/- SEM). The recovery in the other fluids was less than 50% but in glass tubes it increased to 96% after addition of BSA. Binding was also found to the dialysate bag, therefore reducing the amount available for absorption. We recommend that EPO samples from the dialysate are drawn within BSA coated glass tubes

AB - In vitro experiments were performed to analyze problems with the determination of erythropoietin in dialysate. Human recombinant erythropoietin (EPO; 4000 U/L) was added to several fluids, to glass or polystyrene tubes with or without addition of bovine serum albumin (BSA) and to dialysate bags. The recovery in peritoneal effluent was 4120 +/- 203 U/L (mean +/- SEM). The recovery in the other fluids was less than 50% but in glass tubes it increased to 96% after addition of BSA. Binding was also found to the dialysate bag, therefore reducing the amount available for absorption. We recommend that EPO samples from the dialysate are drawn within BSA coated glass tubes

M3 - Article

C2 - 1982829

SN - 1197-8554

VL - 6

SP - 289

EP - 291

JO - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis

JF - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis

ER -