Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

R.D. Fontijn; J. Favre; B.A. Naaijkens; E. Meinster; N.J. Paauw; S.L. Ragghoe; T.D. Nauta; M.A. van den Broek; E.M. Weijers; H.W.M. Niessen; P. Koolwijk; A.J.G. Horrevoets

doi:https://doi.org/10.1016/j.scr.2014.09.004

Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

R.D. Fontijn, J. Favre, B.A. Naaijkens, E. Meinster, N.J. Paauw, S.L. Ragghoe, T.D. Nauta, M.A. van den Broek, E.M. Weijers, H.W.M. Niessen, P. Koolwijk, A.J.G. Horrevoets

Research output: Contribution to journal › Article › Academic › peer-review

16 Citations (Scopus)

Abstract

Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

Original language	English
Pages (from-to)	367-378
Number of pages	12
Journal	Stem Cell Research
Volume	13
Issue number	3 Pt A
DOIs	https://doi.org/10.1016/j.scr.2014.09.004
Publication status	Published - Nov 2014

Keywords

Adipose Tissue
Cell Differentiation
Cell Movement
Cells, Cultured
Cellular Reprogramming
Chemotaxis
Endothelial Cells
Genetic Vectors
Human Umbilical Vein Endothelial Cells
Humans
Journal Article
Matrix Metalloproteinase 7
Microtubules
Research Support, Non-U.S. Gov't
SOXF Transcription Factors
Shear Strength
Stromal Cells
Vascular Endothelial Growth Factor A

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https://doi.org/10.1016/j.scr.2014.09.004

Cite this

Fontijn, R. D., Favre, J., Naaijkens, B. A., Meinster, E., Paauw, N. J., Ragghoe, S. L., Nauta, T. D., van den Broek, M. A., Weijers, E. M., Niessen, H. W. M., Koolwijk, P., & Horrevoets, A. J. G. (2014). Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18. Stem Cell Research, 13(3 Pt A), 367-378. https://doi.org/10.1016/j.scr.2014.09.004

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title = "Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18",

abstract = "Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.",

keywords = "Adipose Tissue, Cell Differentiation, Cell Movement, Cells, Cultured, Cellular Reprogramming, Chemotaxis, Endothelial Cells, Genetic Vectors, Human Umbilical Vein Endothelial Cells, Humans, Journal Article, Matrix Metalloproteinase 7, Microtubules, Research Support, Non-U.S. Gov't, SOXF Transcription Factors, Shear Strength, Stromal Cells, Vascular Endothelial Growth Factor A",

author = "R.D. Fontijn and J. Favre and B.A. Naaijkens and E. Meinster and N.J. Paauw and S.L. Ragghoe and T.D. Nauta and {van den Broek}, M.A. and E.M. Weijers and H.W.M. Niessen and P. Koolwijk and A.J.G. Horrevoets",

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AU - Fontijn, R.D.

AU - Favre, J.

AU - Naaijkens, B.A.

AU - Meinster, E.

AU - Paauw, N.J.

AU - Ragghoe, S.L.

AU - Nauta, T.D.

AU - van den Broek, M.A.

AU - Weijers, E.M.

AU - Niessen, H.W.M.

AU - Koolwijk, P.

AU - Horrevoets, A.J.G.

PY - 2014/11

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N2 - Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

AB - Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

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KW - Cell Movement

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - Chemotaxis

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KW - Genetic Vectors

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Journal Article

KW - Matrix Metalloproteinase 7

KW - Microtubules

KW - Research Support, Non-U.S. Gov't

KW - SOXF Transcription Factors

KW - Shear Strength

KW - Stromal Cells

KW - Vascular Endothelial Growth Factor A

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DO - https://doi.org/10.1016/j.scr.2014.09.004

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JO - Stem Cell Research

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ER -