TY - JOUR
T1 - Aging-regulated anti-apoptotic long non-coding RNA Sarrah augments recovery from acute myocardial infarction
AU - Trembinski, D. Julia
AU - Bink, Diewertje I.
AU - Theodorou, Kosta
AU - Sommer, Janina
AU - Fischer, Ariane
AU - van Bergen, Anke
AU - Kuo, Chao Chung
AU - Costa, Ivan G.
AU - Schürmann, Christoph
AU - Leisegang, Matthias S.
AU - Brandes, Ralf P.
AU - Alekseeva, Tijna
AU - Brill, Boris
AU - Wietelmann, Astrid
AU - Johnson, Christopher N.
AU - Spring-Connell, Alexander
AU - Kaulich, Manuel
AU - Werfel, Stanislas
AU - Engelhardt, Stefan
AU - Hirt, Marc N.
AU - Yorgan, Kaja
AU - Eschenhagen, Thomas
AU - Kirchhof, Luisa
AU - Hofmann, Patrick
AU - Jaé, Nicolas
AU - Wittig, Ilka
AU - Hamdani, Nazha
AU - Bischof, Corinne
AU - Krishnan, Jaya
AU - Houtkooper, Riekelt H.
AU - Dimmeler, Stefanie
AU - Boon, Reinier A.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Long non-coding RNAs (lncRNAs) contribute to cardiac (patho)physiology. Aging is the major risk factor for cardiovascular disease with cardiomyocyte apoptosis as one underlying cause. Here, we report the identification of the aging-regulated lncRNA Sarrah (ENSMUST00000140003) that is anti-apoptotic in cardiomyocytes. Importantly, loss of SARRAH (OXCT1-AS1) in human engineered heart tissue results in impaired contractile force development. SARRAH directly binds to the promoters of genes downregulated after SARRAH silencing via RNA-DNA triple helix formation and cardiomyocytes lacking the triple helix forming domain of Sarrah show an increase in apoptosis. One of the direct SARRAH targets is NRF2, and restoration of NRF2 levels after SARRAH silencing partially rescues the reduction in cell viability. Overexpression of Sarrah in mice shows better recovery of cardiac contractile function after AMI compared to control mice. In summary, we identified the anti-apoptotic evolutionary conserved lncRNA Sarrah, which is downregulated by aging, as a regulator of cardiomyocyte survival.
AB - Long non-coding RNAs (lncRNAs) contribute to cardiac (patho)physiology. Aging is the major risk factor for cardiovascular disease with cardiomyocyte apoptosis as one underlying cause. Here, we report the identification of the aging-regulated lncRNA Sarrah (ENSMUST00000140003) that is anti-apoptotic in cardiomyocytes. Importantly, loss of SARRAH (OXCT1-AS1) in human engineered heart tissue results in impaired contractile force development. SARRAH directly binds to the promoters of genes downregulated after SARRAH silencing via RNA-DNA triple helix formation and cardiomyocytes lacking the triple helix forming domain of Sarrah show an increase in apoptosis. One of the direct SARRAH targets is NRF2, and restoration of NRF2 levels after SARRAH silencing partially rescues the reduction in cell viability. Overexpression of Sarrah in mice shows better recovery of cardiac contractile function after AMI compared to control mice. In summary, we identified the anti-apoptotic evolutionary conserved lncRNA Sarrah, which is downregulated by aging, as a regulator of cardiomyocyte survival.
UR - http://www.scopus.com/inward/record.url?scp=85083980639&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41467-020-15995-2
DO - https://doi.org/10.1038/s41467-020-15995-2
M3 - Article
C2 - 32341350
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2039
ER -