Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma

M. Rihl; J. Gu; D. Baeten; E. Märker-Hermann; J. C. Goodall; J. S. H. Gaston; J. G. Kuipers; H. Zeidler; D. T. Y. Yu

doi:https://doi.org/10.1136/ard.2003.018192

Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma

M. Rihl, J. Gu, D. Baeten, E. Märker-Hermann, J. C. Goodall, J. S. H. Gaston, J. G. Kuipers, H. Zeidler, D. T. Y. Yu

Clinical Immunology and Rheumatology (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

OBJECTIVE: To compare the cytokine expression profile of three CD8+, three CD4+, and three gammadelta+ T cell clones all derived from the synovial fluids of three patients with reactive arthritis (ReA). METHODS: Complementary DNA based microarrays containing the specific sequence of 56 cytokine transcripts were used for screening. Selected genes were confirmed by reverse transcriptase-polymerase chain reaction assay. RESULTS: Microarray showed that transcripts encoding for interferon gamma and tumour necrosis factor alpha were expressed by all CD8+ and CD4+ T cell clones. However, gammadelta+ T cells predominantly expressed transforming growth factor beta2 and granulocyte monocyte-colony stimulating factor. CONCLUSION: T lymphocyte clones from the joint of patients with ReA exhibit differential cytokine expression profiles. CD8+ and CD4+ T cells demonstrate a Th1 mediated profile, whereas gammadelta+ T cells show a more heterogeneous and less proinflammatory Th3 driven pattern

Original language	English
Pages (from-to)	1673-1676
Journal	Annals of the rheumatic diseases
Volume	63
Issue number	12
DOIs	https://doi.org/10.1136/ard.2003.018192
Publication status	Published - 2004

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https://doi.org/10.1136/ard.2003.018192

Cite this

Rihl, M., Gu, J., Baeten, D., Märker-Hermann, E., Goodall, J. C., Gaston, J. S. H., Kuipers, J. G., Zeidler, H., & Yu, D. T. Y. (2004). Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma. Annals of the rheumatic diseases, 63(12), 1673-1676. https://doi.org/10.1136/ard.2003.018192

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title = "Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma",

abstract = "OBJECTIVE: To compare the cytokine expression profile of three CD8+, three CD4+, and three gammadelta+ T cell clones all derived from the synovial fluids of three patients with reactive arthritis (ReA). METHODS: Complementary DNA based microarrays containing the specific sequence of 56 cytokine transcripts were used for screening. Selected genes were confirmed by reverse transcriptase-polymerase chain reaction assay. RESULTS: Microarray showed that transcripts encoding for interferon gamma and tumour necrosis factor alpha were expressed by all CD8+ and CD4+ T cell clones. However, gammadelta+ T cells predominantly expressed transforming growth factor beta2 and granulocyte monocyte-colony stimulating factor. CONCLUSION: T lymphocyte clones from the joint of patients with ReA exhibit differential cytokine expression profiles. CD8+ and CD4+ T cells demonstrate a Th1 mediated profile, whereas gammadelta+ T cells show a more heterogeneous and less proinflammatory Th3 driven pattern",

author = "M. Rihl and J. Gu and D. Baeten and E. M{\"a}rker-Hermann and Goodall, {J. C.} and Gaston, {J. S. H.} and Kuipers, {J. G.} and H. Zeidler and Yu, {D. T. Y.}",

year = "2004",

doi = "https://doi.org/10.1136/ard.2003.018192",

language = "English",

volume = "63",

pages = "1673--1676",

journal = "Annals of the rheumatic diseases",

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Rihl, M, Gu, J, Baeten, D, Märker-Hermann, E, Goodall, JC, Gaston, JSH, Kuipers, JG, Zeidler, H & Yu, DTY 2004, 'Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma', Annals of the rheumatic diseases, vol. 63, no. 12, pp. 1673-1676. https://doi.org/10.1136/ard.2003.018192

Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma. / Rihl, M.; Gu, J.; Baeten, D. et al.
In: Annals of the rheumatic diseases, Vol. 63, No. 12, 2004, p. 1673-1676.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Alpha beta but not gamma delta T cell clones in synovial fluids of patients with reactive arthritis show active transcription of tumour necrosis factor alpha and interferon gamma

AU - Rihl, M.

AU - Gu, J.

AU - Baeten, D.

AU - Märker-Hermann, E.

AU - Goodall, J. C.

AU - Gaston, J. S. H.

AU - Kuipers, J. G.

AU - Zeidler, H.

AU - Yu, D. T. Y.

PY - 2004

Y1 - 2004

N2 - OBJECTIVE: To compare the cytokine expression profile of three CD8+, three CD4+, and three gammadelta+ T cell clones all derived from the synovial fluids of three patients with reactive arthritis (ReA). METHODS: Complementary DNA based microarrays containing the specific sequence of 56 cytokine transcripts were used for screening. Selected genes were confirmed by reverse transcriptase-polymerase chain reaction assay. RESULTS: Microarray showed that transcripts encoding for interferon gamma and tumour necrosis factor alpha were expressed by all CD8+ and CD4+ T cell clones. However, gammadelta+ T cells predominantly expressed transforming growth factor beta2 and granulocyte monocyte-colony stimulating factor. CONCLUSION: T lymphocyte clones from the joint of patients with ReA exhibit differential cytokine expression profiles. CD8+ and CD4+ T cells demonstrate a Th1 mediated profile, whereas gammadelta+ T cells show a more heterogeneous and less proinflammatory Th3 driven pattern

AB - OBJECTIVE: To compare the cytokine expression profile of three CD8+, three CD4+, and three gammadelta+ T cell clones all derived from the synovial fluids of three patients with reactive arthritis (ReA). METHODS: Complementary DNA based microarrays containing the specific sequence of 56 cytokine transcripts were used for screening. Selected genes were confirmed by reverse transcriptase-polymerase chain reaction assay. RESULTS: Microarray showed that transcripts encoding for interferon gamma and tumour necrosis factor alpha were expressed by all CD8+ and CD4+ T cell clones. However, gammadelta+ T cells predominantly expressed transforming growth factor beta2 and granulocyte monocyte-colony stimulating factor. CONCLUSION: T lymphocyte clones from the joint of patients with ReA exhibit differential cytokine expression profiles. CD8+ and CD4+ T cells demonstrate a Th1 mediated profile, whereas gammadelta+ T cells show a more heterogeneous and less proinflammatory Th3 driven pattern

U2 - https://doi.org/10.1136/ard.2003.018192

DO - https://doi.org/10.1136/ard.2003.018192

M3 - Article

C2 - 15547094

SN - 0003-4967

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JO - Annals of the rheumatic diseases

JF - Annals of the rheumatic diseases

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