Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure

Georgios Kosmidis; Milena Bellin; Marcelo C. Ribeiro; Berend van Meer; Dorien Ward-van Oostwaard; Robert Passier; Leon G. J. Tertoolen; Christine L. Mummery; Simona Casini

doi:https://doi.org/10.1016/j.bbrc.2015.10.026

Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure

Georgios Kosmidis, Milena Bellin, Marcelo C. Ribeiro, Berend van Meer, Dorien Ward-van Oostwaard, Robert Passier, Leon G. J. Tertoolen, Christine L. Mummery, Simona Casini

Cardiology (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

One limitation in using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) for disease modeling and cardiac safety pharmacology is their immature functional phenotype compared with adult cardiomyocytes. Here, we report that treatment of human embryonic stem cell derived cardiomyocytes (hESC-CMs) with dexamethasone, a synthetic glucocorticoid, activated glucocorticoid signaling which in turn improved their calcium handling properties and contractility. L-type calcium current and action potential properties were not affected by dexamethasone but significantly faster calcium decay, increased forces of contraction and sarcomeric lengths, were observed in hESC-CMs after dexamethasone exposure. Activating the glucocorticoid pathway can thus contribute to mediating hPSC-CMs maturation

Original language	English
Pages (from-to)	998-1005
Journal	Biochemical and Biophysical Research Communications
Volume	467
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2015.10.026
Publication status	Published - 2015

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https://doi.org/10.1016/j.bbrc.2015.10.026

Cite this

Kosmidis, G., Bellin, M., Ribeiro, M. C., van Meer, B., Ward-van Oostwaard, D., Passier, R., Tertoolen, L. G. J., Mummery, C. L., & Casini, S. (2015). Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure. Biochemical and Biophysical Research Communications, 467(4), 998-1005. https://doi.org/10.1016/j.bbrc.2015.10.026

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title = "Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure",

abstract = "One limitation in using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) for disease modeling and cardiac safety pharmacology is their immature functional phenotype compared with adult cardiomyocytes. Here, we report that treatment of human embryonic stem cell derived cardiomyocytes (hESC-CMs) with dexamethasone, a synthetic glucocorticoid, activated glucocorticoid signaling which in turn improved their calcium handling properties and contractility. L-type calcium current and action potential properties were not affected by dexamethasone but significantly faster calcium decay, increased forces of contraction and sarcomeric lengths, were observed in hESC-CMs after dexamethasone exposure. Activating the glucocorticoid pathway can thus contribute to mediating hPSC-CMs maturation",

author = "Georgios Kosmidis and Milena Bellin and Ribeiro, {Marcelo C.} and {van Meer}, Berend and {Ward-van Oostwaard}, Dorien and Robert Passier and Tertoolen, {Leon G. J.} and Mummery, {Christine L.} and Simona Casini",

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Kosmidis, G, Bellin, M, Ribeiro, MC, van Meer, B, Ward-van Oostwaard, D, Passier, R, Tertoolen, LGJ, Mummery, CL & Casini, S 2015, 'Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure', Biochemical and Biophysical Research Communications, vol. 467, no. 4, pp. 998-1005. https://doi.org/10.1016/j.bbrc.2015.10.026

Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure. / Kosmidis, Georgios; Bellin, Milena; Ribeiro, Marcelo C. et al.
In: Biochemical and Biophysical Research Communications, Vol. 467, No. 4, 2015, p. 998-1005.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Altered calcium handling and increased contraction force in human embryonic stem cell derived cardiomyocytes following short term dexamethasone exposure

AU - Kosmidis, Georgios

AU - Bellin, Milena

AU - Ribeiro, Marcelo C.

AU - van Meer, Berend

AU - Ward-van Oostwaard, Dorien

AU - Passier, Robert

AU - Tertoolen, Leon G. J.

AU - Mummery, Christine L.

AU - Casini, Simona

PY - 2015

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N2 - One limitation in using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) for disease modeling and cardiac safety pharmacology is their immature functional phenotype compared with adult cardiomyocytes. Here, we report that treatment of human embryonic stem cell derived cardiomyocytes (hESC-CMs) with dexamethasone, a synthetic glucocorticoid, activated glucocorticoid signaling which in turn improved their calcium handling properties and contractility. L-type calcium current and action potential properties were not affected by dexamethasone but significantly faster calcium decay, increased forces of contraction and sarcomeric lengths, were observed in hESC-CMs after dexamethasone exposure. Activating the glucocorticoid pathway can thus contribute to mediating hPSC-CMs maturation

AB - One limitation in using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) for disease modeling and cardiac safety pharmacology is their immature functional phenotype compared with adult cardiomyocytes. Here, we report that treatment of human embryonic stem cell derived cardiomyocytes (hESC-CMs) with dexamethasone, a synthetic glucocorticoid, activated glucocorticoid signaling which in turn improved their calcium handling properties and contractility. L-type calcium current and action potential properties were not affected by dexamethasone but significantly faster calcium decay, increased forces of contraction and sarcomeric lengths, were observed in hESC-CMs after dexamethasone exposure. Activating the glucocorticoid pathway can thus contribute to mediating hPSC-CMs maturation

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