An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

C. van Oven; P.M. Krawczyk; J. Stap; A.M. Melo; M.H.O. Piazzetta; A.L. Gobbi; H.A. van Veen; J. Verhoeven; J.A. Aten

doi:https://doi.org/10.1007/s00249-009-0472-7

An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

C. van Oven, P.M. Krawczyk, J. Stap, A.M. Melo, M.H.O. Piazzetta, A.L. Gobbi, H.A. van Veen, J. Verhoeven, J.A. Aten

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

Original language	English
Pages (from-to)	721-728
Journal	European Biophysics Journal with Biophysics Letters
Volume	38
Issue number	6
DOIs	https://doi.org/10.1007/s00249-009-0472-7
Publication status	Published - 2009

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https://doi.org/10.1007/s00249-009-0472-7

https://pure.uva.nl/ws/files/802329/78734_322280.pdf

Cite this

van Oven, C., Krawczyk, P. M., Stap, J., Melo, A. M., Piazzetta, M. H. O., Gobbi, A. L., van Veen, H. A., Verhoeven, J., & Aten, J. A. (2009). An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy. European Biophysics Journal with Biophysics Letters, 38(6), 721-728. https://doi.org/10.1007/s00249-009-0472-7

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van Oven, C, Krawczyk, PM, Stap, J, Melo, AM, Piazzetta, MHO, Gobbi, AL, van Veen, HA, Verhoeven, J & Aten, JA 2009, 'An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy', European Biophysics Journal with Biophysics Letters, vol. 38, no. 6, pp. 721-728. https://doi.org/10.1007/s00249-009-0472-7

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AU - van Oven, C.

AU - Krawczyk, P.M.

AU - Stap, J.

AU - Melo, A.M.

AU - Piazzetta, M.H.O.

AU - Gobbi, A.L.

AU - van Veen, H.A.

AU - Verhoeven, J.

AU - Aten, J.A.

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AB - Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

U2 - https://doi.org/10.1007/s00249-009-0472-7

DO - https://doi.org/10.1007/s00249-009-0472-7

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