TY - JOUR
T1 - An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells
AU - Herzog, Katharina
AU - Ijlst, Lodewijk
AU - van Cruchten, Arno G.
AU - van Roermund, Carlo W. T.
AU - Kulik, Wim
AU - Wanders, Ronald J. A.
AU - Waterham, Hans R.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
AB - Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064521253&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30841653
U2 - https://doi.org/10.3390/metabo9030045
DO - https://doi.org/10.3390/metabo9030045
M3 - Article
C2 - 30841653
SN - 2218-1989
VL - 9
JO - Metabolites
JF - Metabolites
IS - 3
M1 - 45
ER -