Analysis of 6-mercaptopurine in human plasma with a high-performance liquid chromatographic method including post-column derivatization and fluorimetric detection

R. E. Jonkers; B. Oosterhuis; R. J. ten Berge; C. J. van Boxtel

doi:https://doi.org/10.1016/S0378-4347(00)81751-X

Analysis of 6-mercaptopurine in human plasma with a high-performance liquid chromatographic method including post-column derivatization and fluorimetric detection

R. E. Jonkers, B. Oosterhuis, R. J. ten Berge, C. J. van Boxtel

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

A relatively simple assay with improved reliability and sensitivity for measuring levels of 6-mercaptopurine in human plasma is presented. After extraction of the compound and the added internal standard with phenyl mercury acetate, samples were separated by ion-pair reversed-phase high-performance liquid chromatography. On-line the analytes were oxidized to fluorescent products and detected in a flow-fluorimeter. The within-day coefficient of variation was 3.8% at a concentration of 25 ng/ml. The lower detection limit was 2 ng/ml when 1.0 ml of plasma was used. Mercaptopurine concentration versus time curves of two subjects after a single oral dose of azathioprine are shown

Original language	English
Pages (from-to)	249-255
Journal	Journal of chromatography. A
Volume	233
DOIs	https://doi.org/10.1016/S0378-4347(00)81751-X
Publication status	Published - 1982

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https://doi.org/10.1016/S0378-4347(00)81751-X

Cite this

@article{e0652ec87ff44735a41167d17d88dc46,

title = "Analysis of 6-mercaptopurine in human plasma with a high-performance liquid chromatographic method including post-column derivatization and fluorimetric detection",

abstract = "A relatively simple assay with improved reliability and sensitivity for measuring levels of 6-mercaptopurine in human plasma is presented. After extraction of the compound and the added internal standard with phenyl mercury acetate, samples were separated by ion-pair reversed-phase high-performance liquid chromatography. On-line the analytes were oxidized to fluorescent products and detected in a flow-fluorimeter. The within-day coefficient of variation was 3.8% at a concentration of 25 ng/ml. The lower detection limit was 2 ng/ml when 1.0 ml of plasma was used. Mercaptopurine concentration versus time curves of two subjects after a single oral dose of azathioprine are shown",

author = "Jonkers, {R. E.} and B. Oosterhuis and {ten Berge}, {R. J.} and {van Boxtel}, {C. J.}",

year = "1982",

doi = "https://doi.org/10.1016/S0378-4347(00)81751-X",

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pages = "249--255",

journal = "Journal of chromatography. A",

issn = "0021-9673",

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AU - Jonkers, R. E.

AU - Oosterhuis, B.

AU - ten Berge, R. J.

AU - van Boxtel, C. J.

PY - 1982

Y1 - 1982

N2 - A relatively simple assay with improved reliability and sensitivity for measuring levels of 6-mercaptopurine in human plasma is presented. After extraction of the compound and the added internal standard with phenyl mercury acetate, samples were separated by ion-pair reversed-phase high-performance liquid chromatography. On-line the analytes were oxidized to fluorescent products and detected in a flow-fluorimeter. The within-day coefficient of variation was 3.8% at a concentration of 25 ng/ml. The lower detection limit was 2 ng/ml when 1.0 ml of plasma was used. Mercaptopurine concentration versus time curves of two subjects after a single oral dose of azathioprine are shown

AB - A relatively simple assay with improved reliability and sensitivity for measuring levels of 6-mercaptopurine in human plasma is presented. After extraction of the compound and the added internal standard with phenyl mercury acetate, samples were separated by ion-pair reversed-phase high-performance liquid chromatography. On-line the analytes were oxidized to fluorescent products and detected in a flow-fluorimeter. The within-day coefficient of variation was 3.8% at a concentration of 25 ng/ml. The lower detection limit was 2 ng/ml when 1.0 ml of plasma was used. Mercaptopurine concentration versus time curves of two subjects after a single oral dose of azathioprine are shown

U2 - https://doi.org/10.1016/S0378-4347(00)81751-X

DO - https://doi.org/10.1016/S0378-4347(00)81751-X

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C2 - 6891704

SN - 0021-9673

VL - 233

SP - 249

EP - 255

JO - Journal of chromatography. A

JF - Journal of chromatography. A

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