Androgens down-regulate myosin light chain kinase in human prostate cancer cells

Nicolas Léveillé; Andréa Fournier; Claude Labrie

doi:https://doi.org/10.1016/j.jsbmb.2009.02.002

Androgens down-regulate myosin light chain kinase in human prostate cancer cells

Nicolas Léveillé, Andréa Fournier, Claude Labrie

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Androgens play a major role in the growth and survival of primary prostate tumors. The molecular mechanisms involved in prostate cancer progression are not fully understood but genes that are regulated by androgens clearly influence this process. We searched for new androgen-regulated genes using the Affymetrix GeneChip Human Genome U95 Set in the androgen-sensitive LNCaP prostate cancer cell line. Analysis of gene expression profiles revealed that myosin light chain kinase (MLCK) mRNA levels were markedly down-regulated by the synthetic androgen R1881. The microarray data were confirmed by ribonuclease protection assays. RNA and protein analyses revealed that LNCaP cells express both long (non-muscle) and short (smooth muscle) isoforms, and that both isoforms are down-regulated by androgens. Taken together, these data identify MLCK as a novel downstream target of the androgen signalling pathway in prostate cells.

Original language	English
Pages (from-to)	174-179
Number of pages	6
Journal	Journal of Steroid Biochemistry and Molecular Biology
Volume	114
Issue number	3-5
DOIs	https://doi.org/10.1016/j.jsbmb.2009.02.002
Publication status	Published - Apr 2009

Keywords

Androgens
MLCK
Prostate cancer

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https://doi.org/10.1016/j.jsbmb.2009.02.002

Cite this

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title = "Androgens down-regulate myosin light chain kinase in human prostate cancer cells",

abstract = "Androgens play a major role in the growth and survival of primary prostate tumors. The molecular mechanisms involved in prostate cancer progression are not fully understood but genes that are regulated by androgens clearly influence this process. We searched for new androgen-regulated genes using the Affymetrix GeneChip Human Genome U95 Set in the androgen-sensitive LNCaP prostate cancer cell line. Analysis of gene expression profiles revealed that myosin light chain kinase (MLCK) mRNA levels were markedly down-regulated by the synthetic androgen R1881. The microarray data were confirmed by ribonuclease protection assays. RNA and protein analyses revealed that LNCaP cells express both long (non-muscle) and short (smooth muscle) isoforms, and that both isoforms are down-regulated by androgens. Taken together, these data identify MLCK as a novel downstream target of the androgen signalling pathway in prostate cells.",

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AU - Léveillé, Nicolas

AU - Fournier, Andréa

AU - Labrie, Claude

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AB - Androgens play a major role in the growth and survival of primary prostate tumors. The molecular mechanisms involved in prostate cancer progression are not fully understood but genes that are regulated by androgens clearly influence this process. We searched for new androgen-regulated genes using the Affymetrix GeneChip Human Genome U95 Set in the androgen-sensitive LNCaP prostate cancer cell line. Analysis of gene expression profiles revealed that myosin light chain kinase (MLCK) mRNA levels were markedly down-regulated by the synthetic androgen R1881. The microarray data were confirmed by ribonuclease protection assays. RNA and protein analyses revealed that LNCaP cells express both long (non-muscle) and short (smooth muscle) isoforms, and that both isoforms are down-regulated by androgens. Taken together, these data identify MLCK as a novel downstream target of the androgen signalling pathway in prostate cells.

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Androgens down-regulate myosin light chain kinase in human prostate cancer cells

Abstract

Keywords

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