TY - JOUR
T1 - Annexin A5-conjugated quantum dots with a paramagnetic lipidic coating for the multimodal detection of apoptotic cells
AU - van Tilborg, Geralda A. F.
AU - Mulder, Willem J. M.
AU - Chin, Patrick T. K.
AU - Storm, Gert
AU - Reutelingsperger, Chris P.
AU - Nicolay, Klaas
AU - Strijkers, Gustav J.
PY - 2006
Y1 - 2006
N2 - Apoptosis, or programmed cell death, plays an important role in the etiology of a variety of diseases, including cancer. Visualization of apoptosis would allow both early detection of therapy efficiency and evaluation of disease progression. To that aim we developed a novel annexin A5-conjugated bimodal nanoparticle. The nanoparticle is composed of a quantum dot that is encapsulated in a paramagnetic micelle to enable its use both for optical imaging and MRI. Multiple recombinant human annexin A5 protein molecules were covalently coupled to the nanoparticle for targeting. In this study the specificity of the annexin A5-conjugated nanoparticles for apoptotic cells was demonstrated both with fluorescence microscopy and MRI, which confirms its potential for the detection of apoptosis with both imaging modalities in vivo
AB - Apoptosis, or programmed cell death, plays an important role in the etiology of a variety of diseases, including cancer. Visualization of apoptosis would allow both early detection of therapy efficiency and evaluation of disease progression. To that aim we developed a novel annexin A5-conjugated bimodal nanoparticle. The nanoparticle is composed of a quantum dot that is encapsulated in a paramagnetic micelle to enable its use both for optical imaging and MRI. Multiple recombinant human annexin A5 protein molecules were covalently coupled to the nanoparticle for targeting. In this study the specificity of the annexin A5-conjugated nanoparticles for apoptotic cells was demonstrated both with fluorescence microscopy and MRI, which confirms its potential for the detection of apoptosis with both imaging modalities in vivo
U2 - https://doi.org/10.1021/bc0600463
DO - https://doi.org/10.1021/bc0600463
M3 - Article
C2 - 16848390
SN - 1043-1802
VL - 17
SP - 865
EP - 868
JO - Bioconjugate chemistry
JF - Bioconjugate chemistry
IS - 4
ER -