Apolipoprotein E2/E3/E4 genotyping with agarose gels

G. M. Dallinga-Thie; M. van Linde-Sibenius Trip; L. A. Kock; T. W. de Bruin

Apolipoprotein E2/E3/E4 genotyping with agarose gels

G. M. Dallinga-Thie, M. van Linde-Sibenius Trip, L. A. Kock, T. W. de Bruin

Other Departments

Research output: Contribution to journal › Article › Academic › peer-review

50 Citations (Scopus)

Abstract

Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles

Original language	English
Pages (from-to)	73-75
Journal	Clinical Chemistry
Volume	41
Issue number	1
Publication status	Published - 1995

Cite this

@article{339e886b247042fd990ebc8674d81f9a,

title = "Apolipoprotein E2/E3/E4 genotyping with agarose gels",

abstract = "Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles",

author = "Dallinga-Thie, {G. M.} and {van Linde-Sibenius Trip}, M. and Kock, {L. A.} and {de Bruin}, {T. W.}",

year = "1995",

language = "English",

volume = "41",

pages = "73--75",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry Inc.",

number = "1",

}

TY - JOUR

T1 - Apolipoprotein E2/E3/E4 genotyping with agarose gels

AU - Dallinga-Thie, G. M.

AU - van Linde-Sibenius Trip, M.

AU - Kock, L. A.

AU - de Bruin, T. W.

PY - 1995

Y1 - 1995

N2 - Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles

AB - Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles

M3 - Article

C2 - 7813084

SN - 0009-9147

VL - 41

SP - 73

EP - 75

JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 1

ER -