Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts

H. L. Smits; B. van Gemen; R. Schukkink; J. van der Velden; S. P. Tjong-A-Hung; M. F. Jebbink; J. ter Schegget

doi:https://doi.org/10.1016/0166-0934(95)00032-P

Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts

H. L. Smits, B. van Gemen, R. Schukkink, J. van der Velden, S. P. Tjong-A-Hung, M. F. Jebbink, J. ter Schegget

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Research output: Contribution to journal › Comment/Letter to the editor › Academic

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Abstract

Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears

Original language	English
Pages (from-to)	75-81
Journal	Journal of virological methods
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(95)00032-P
Publication status	Published - 1995

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https://doi.org/10.1016/0166-0934(95)00032-P

Cite this

@article{34a91f3fa73f4fdc922b9fc4a9f49ee5,

title = "Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts",

abstract = "Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears",

author = "Smits, {H. L.} and {van Gemen}, B. and R. Schukkink and {van der Velden}, J. and Tjong-A-Hung, {S. P.} and Jebbink, {M. F.} and {ter Schegget}, J.",

year = "1995",

doi = "https://doi.org/10.1016/0166-0934(95)00032-P",

language = "English",

volume = "54",

pages = "75--81",

journal = "Journal of virological methods",

issn = "0166-0934",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts

AU - Smits, H. L.

AU - van Gemen, B.

AU - Schukkink, R.

AU - van der Velden, J.

AU - Tjong-A-Hung, S. P.

AU - Jebbink, M. F.

AU - ter Schegget, J.

PY - 1995

Y1 - 1995

N2 - Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears

AB - Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears

U2 - https://doi.org/10.1016/0166-0934(95)00032-P

DO - https://doi.org/10.1016/0166-0934(95)00032-P

M3 - Comment/Letter to the editor

C2 - 7559859

SN - 0166-0934

VL - 54

SP - 75

EP - 81

JO - Journal of virological methods

JF - Journal of virological methods

IS - 1

ER -