TY - JOUR
T1 - Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions
AU - Chornyi, Serhii
AU - Koster, Janet
AU - Waterham, Hans R.
N1 - Funding Information: This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 812968 (PERICO). Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches. Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.
AB - The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches. Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.
KW - CRISPR-Cas9
KW - Gene deletion
KW - Gene disruption
KW - Gene editing
KW - Gene knockout
KW - PEX genes
UR - http://www.scopus.com/inward/record.url?scp=85151043789&partnerID=8YFLogxK
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85151043789&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/36952190
U2 - https://doi.org/10.1007/978-1-0716-3048-8_17
DO - https://doi.org/10.1007/978-1-0716-3048-8_17
M3 - Article
C2 - 36952190
SN - 1064-3745
VL - 2643
SP - 233
EP - 245
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -