Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn; Aswani R. Bolla; Lynn L. Barron; Mercedes E. Gorre; Mansoor S. Mohammed; David W. Bahler; Clemens H. M. Mellink; Marinus H. J. van Oers; Michael J. Keating; Alessandra Ferrajoli; Kevin R. Coombes; Lynne V. Abruzzo; Ryan S. Robetorye

doi:https://doi.org/10.1016/j.leukres.2008.10.010

Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn, Aswani R. Bolla, Lynn L. Barron, Mercedes E. Gorre, Mansoor S. Mohammed, David W. Bahler, Clemens H. M. Mellink, Marinus H. J. van Oers, Michael J. Keating, Alessandra Ferrajoli, Kevin R. Coombes, Lynne V. Abruzzo, Ryan S. Robetorye

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Abstract

We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34Mb up to approximately 1Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases

Original language	English
Pages (from-to)	1276-1281
Journal	Leukemia research
Volume	33
Issue number	9
DOIs	https://doi.org/10.1016/j.leukres.2008.10.010
Publication status	Published - 2009

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https://doi.org/10.1016/j.leukres.2008.10.010

Cite this

Gunn, S. R., Bolla, A. R., Barron, L. L., Gorre, M. E., Mohammed, M. S., Bahler, D. W., Mellink, C. H. M., van Oers, M. H. J., Keating, M. J., Ferrajoli, A., Coombes, K. R., Abruzzo, L. V., & Robetorye, R. S. (2009). Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene. Leukemia research, 33(9), 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010

@article{18acffa87ae444eeae3725af10b5d98b,

title = "Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene",

abstract = "We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34Mb up to approximately 1Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases",

author = "Gunn, {Shelly R.} and Bolla, {Aswani R.} and Barron, {Lynn L.} and Gorre, {Mercedes E.} and Mohammed, {Mansoor S.} and Bahler, {David W.} and Mellink, {Clemens H. M.} and {van Oers}, {Marinus H. J.} and Keating, {Michael J.} and Alessandra Ferrajoli and Coombes, {Kevin R.} and Abruzzo, {Lynne V.} and Robetorye, {Ryan S.}",

year = "2009",

doi = "https://doi.org/10.1016/j.leukres.2008.10.010",

language = "English",

volume = "33",

pages = "1276--1281",

journal = "Leukemia research",

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publisher = "Elsevier Limited",

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Gunn, SR, Bolla, AR, Barron, LL, Gorre, ME, Mohammed, MS, Bahler, DW, Mellink, CHM , van Oers, MHJ, Keating, MJ, Ferrajoli, A, Coombes, KR, Abruzzo, LV & Robetorye, RS 2009, 'Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene', Leukemia research, vol. 33, no. 9, pp. 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010

TY - JOUR

T1 - Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

AU - Gunn, Shelly R.

AU - Bolla, Aswani R.

AU - Barron, Lynn L.

AU - Gorre, Mercedes E.

AU - Mohammed, Mansoor S.

AU - Bahler, David W.

AU - Mellink, Clemens H. M.

AU - van Oers, Marinus H. J.

AU - Keating, Michael J.

AU - Ferrajoli, Alessandra

AU - Coombes, Kevin R.

AU - Abruzzo, Lynne V.

AU - Robetorye, Ryan S.

PY - 2009

Y1 - 2009

N2 - We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34Mb up to approximately 1Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases

AB - We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34Mb up to approximately 1Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases

U2 - https://doi.org/10.1016/j.leukres.2008.10.010

DO - https://doi.org/10.1016/j.leukres.2008.10.010

M3 - Article

C2 - 19027161

SN - 0145-2126

VL - 33

SP - 1276

EP - 1281

JO - Leukemia research

JF - Leukemia research

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ER -