TY - JOUR
T1 - Artificial reporter gene providing MRI contrast based on proton exchange
AU - Gilad, Assaf A.
AU - McMahon, Michael T.
AU - Walczak, Piotr
AU - Winnard, Paul T.
AU - Raman, Venu
AU - van Laarhoven, Hanneke W. M.
AU - Skoglund, Cynthia M.
AU - Bulte, Jeff W. M.
AU - van Zijl, Peter C. M.
PY - 2007
Y1 - 2007
N2 - Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off
AB - Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off
U2 - https://doi.org/10.1038/nbt1277
DO - https://doi.org/10.1038/nbt1277
M3 - Article
C2 - 17259977
SN - 1087-0156
VL - 25
SP - 217
EP - 219
JO - Nature biotechnology
JF - Nature biotechnology
IS - 2
ER -