TY - JOUR
T1 - Artificial Sporulation Induction (ASI) by kinA Overexpression Affects the Proteomes and Properties of Bacillus subtilis Spores
AU - Tu, Z.
AU - Abhyankar, W.R.
AU - Swarge, B.N.
AU - van der Wel, N.
AU - Kramer, G.
AU - Brul, S.
AU - de Koning, L.J.
N1 - With supplementary file
PY - 2020/6/2
Y1 - 2020/6/2
N2 - To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of synchronized sporulation initiation on spore resistance, structures, the germination behavior at single-spore level and the proteome. The results indicate that, in our set up, the sporulation by overexpressing kinA can generate a spore yield of 70% within 8 h. The procedure increases spore wet heat resistance and thickness of the spore coat and cortex layers, whilst delaying the time to spore phase-darkening and burst after addition of germinant. The proteome analysis reveals that the upregulated proteins in the kinA induced spores, compared to spores without kinA induction, as well as the 'wildtype' spores, are mostly involved in spore formation. The downregulated proteins mostly belong to the categories of coping with stress, carbon and nitrogen metabolism, as well as the regulation of sporulation. Thus, while kinA overexpression enhances synchronicity in sporulation initiation, it also has profound effects on the central equilibrium of spore formation and spore germination, through modulation of the spore molecular composition and stress resistance physiology.
AB - To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of synchronized sporulation initiation on spore resistance, structures, the germination behavior at single-spore level and the proteome. The results indicate that, in our set up, the sporulation by overexpressing kinA can generate a spore yield of 70% within 8 h. The procedure increases spore wet heat resistance and thickness of the spore coat and cortex layers, whilst delaying the time to spore phase-darkening and burst after addition of germinant. The proteome analysis reveals that the upregulated proteins in the kinA induced spores, compared to spores without kinA induction, as well as the 'wildtype' spores, are mostly involved in spore formation. The downregulated proteins mostly belong to the categories of coping with stress, carbon and nitrogen metabolism, as well as the regulation of sporulation. Thus, while kinA overexpression enhances synchronicity in sporulation initiation, it also has profound effects on the central equilibrium of spore formation and spore germination, through modulation of the spore molecular composition and stress resistance physiology.
KW - B. subtilis
KW - kinA induction
KW - spore proteomics
KW - sporulation homogeneity
UR - http://www.scopus.com/inward/record.url?scp=85086753326&partnerID=8YFLogxK
UR - https://pure.uva.nl/ws/files/49951099/ijms_21_04315_s001.zip
U2 - https://doi.org/10.3390/ijms21124315
DO - https://doi.org/10.3390/ijms21124315
M3 - Article
C2 - 32560401
SN - 1661-6596
VL - 21
SP - 1
EP - 18
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 12
M1 - 4315
ER -