Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein

Gerco den Hartog; Stijn P. Andeweg; Christina E. Hoeve; Gaby Smits; Bettie Voordouw; Dirk Eggink; Mirjam J. Knol; Robert S. van Binnendijk

doi:https://doi.org/10.1038/s41598-023-45718-8

Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein

Gerco den Hartog, Stijn P. Andeweg, Christina E. Hoeve, Gaby Smits, Bettie Voordouw, Dirk Eggink, Mirjam J. Knol, Robert S. van Binnendijk

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Immunity induced by vaccination and infection, referred to as hybrid immunity, provides better protection against SARS-CoV-2 infections compared to immunity induced by vaccinations alone. To assess the development of hybrid immunity we investigated the induction of Nucleoprotein-specific antibodies in PCR-confirmed infections by Delta or Omicron in vaccinated individuals (n = 520). Eighty-two percent of the participants with a breakthrough infection reached N-seropositivity. N-seropositivity was accompanied by Spike S1 antibody boosting, and independent of vaccination status or virus variant. Following the infection relatively more antibodies to the infecting virus variant were detected. In conclusion, these data show that hybrid immunity through breakthrough infections is hallmarked by Nucleoprotein antibodies and broadening of the Spike antibody repertoire. Exposure to future SARS-CoV-2 variants may therefore continue to maintain and broaden vaccine-induced population immunity.

Original language	English
Article number	18394
Journal	Scientific reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-45718-8
Publication status	Published - 1 Dec 2023

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den Hartog, G., Andeweg, S. P., Hoeve, C. E., Smits, G., Voordouw, B., Eggink, D., Knol, M. J., & van Binnendijk, R. S. (2023). Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein. Scientific reports, 13(1), Article 18394. https://doi.org/10.1038/s41598-023-45718-8

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title = "Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein",

abstract = "Immunity induced by vaccination and infection, referred to as hybrid immunity, provides better protection against SARS-CoV-2 infections compared to immunity induced by vaccinations alone. To assess the development of hybrid immunity we investigated the induction of Nucleoprotein-specific antibodies in PCR-confirmed infections by Delta or Omicron in vaccinated individuals (n = 520). Eighty-two percent of the participants with a breakthrough infection reached N-seropositivity. N-seropositivity was accompanied by Spike S1 antibody boosting, and independent of vaccination status or virus variant. Following the infection relatively more antibodies to the infecting virus variant were detected. In conclusion, these data show that hybrid immunity through breakthrough infections is hallmarked by Nucleoprotein antibodies and broadening of the Spike antibody repertoire. Exposure to future SARS-CoV-2 variants may therefore continue to maintain and broaden vaccine-induced population immunity.",

author = "{den Hartog}, Gerco and Andeweg, {Stijn P.} and Hoeve, {Christina E.} and Gaby Smits and Bettie Voordouw and Dirk Eggink and Knol, {Mirjam J.} and {van Binnendijk}, {Robert S.}",

note = "Funding Information: The study was funded by the Dutch Ministry of Health, Welfare and Sports. Funding Information: We thank the laboratory team of the National Institute for Public Health and the Environment (RIVM), Afke Vogelzang, Annemarie van den Brandt, Bas van der Veer, Jeroen Cremer, Jil Kocken, Jordy de Bakker, Kim Freriks, Lisa Wijsman, Lynn Aarts, Ryanne Jaarsma, Sanne Bos and Sharon van den Brink. We thank Teun Guichelaar and Joanna Kaczorowska for critically reviewing the manuscript. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

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Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein. / den Hartog, Gerco; Andeweg, Stijn P.; Hoeve, Christina E. et al.
In: Scientific reports, Vol. 13, No. 1, 18394, 01.12.2023.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Andeweg, Stijn P.

AU - Hoeve, Christina E.

AU - Smits, Gaby

AU - Voordouw, Bettie

AU - Eggink, Dirk

AU - Knol, Mirjam J.

AU - van Binnendijk, Robert S.

N1 - Funding Information: The study was funded by the Dutch Ministry of Health, Welfare and Sports. Funding Information: We thank the laboratory team of the National Institute for Public Health and the Environment (RIVM), Afke Vogelzang, Annemarie van den Brandt, Bas van der Veer, Jeroen Cremer, Jil Kocken, Jordy de Bakker, Kim Freriks, Lisa Wijsman, Lynn Aarts, Ryanne Jaarsma, Sanne Bos and Sharon van den Brink. We thank Teun Guichelaar and Joanna Kaczorowska for critically reviewing the manuscript. Publisher Copyright: © 2023, The Author(s).

PY - 2023/12/1

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Assessment of hybrid population immunity to SARS-CoV-2 following breakthrough infections of distinct SARS-CoV-2 variants by the detection of antibodies to nucleoprotein

Abstract

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