Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes

Ferran Nieto-Fabregat; Qian Zhu; Corinne Vivès; Yunqin Zhang; Angela Marseglia; Fabrizio Chiodo; Michel Thépaut; Diksha Rai; Suvarn S. Kulkarni; Flaviana di Lorenzo; Antonio Molinaro; Roberta Marchetti; Franck Fieschi; Guozhi Xiao; Biao Yu; Alba Silipo

doi:10.1021/jacsau.3c00748

Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes

Ferran Nieto-Fabregat, Qian Zhu, Corinne Vivès, Yunqin Zhang, Angela Marseglia, Fabrizio Chiodo, Michel Thépaut, Diksha Rai, Suvarn S. Kulkarni, Flaviana di Lorenzo, Antonio Molinaro, Roberta Marchetti, Franck Fieschi, Guozhi Xiao, Biao Yu, Alba Silipo

Molecular cell biology and Immunology (VUmc)

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical dissection of BVMPK LPS components, attained by synthetic chemistry combined to spectroscopic, biophysical, and computational techniques, allowed to finely map the LPS epitopes recognized by DC-SIGN. Our findings reveal BVMPK’s role in immune modulation via DC-SIGN, targeting both the LPS O-antigen and the core oligosaccharide. Furthermore, when framed within medical chemistry or drug design, our results could lead to the development of tailored molecules to benefit the hosts dealing with inflammatory diseases.

Original language	English
Pages (from-to)	697-712
Number of pages	16
Journal	JACS AU
Volume	4
Issue number	2
DOIs	https://doi.org/10.1021/jacsau.3c00748
Publication status	Published - 26 Feb 2024

Keywords

Bacteroides vulgatus lipopolysaccharide
DC-SIGN
NMR spectroscopy
gut microbiota
lectins

Access to Document

10.1021/jacsau.3c00748

Cite this

@article{7ef72ab04cba4c019736da66ea648d51,

title = "Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes",

abstract = "The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical dissection of BVMPK LPS components, attained by synthetic chemistry combined to spectroscopic, biophysical, and computational techniques, allowed to finely map the LPS epitopes recognized by DC-SIGN. Our findings reveal BVMPK{\textquoteright}s role in immune modulation via DC-SIGN, targeting both the LPS O-antigen and the core oligosaccharide. Furthermore, when framed within medical chemistry or drug design, our results could lead to the development of tailored molecules to benefit the hosts dealing with inflammatory diseases.",

keywords = "Bacteroides vulgatus lipopolysaccharide, DC-SIGN, NMR spectroscopy, gut microbiota, lectins",

author = "Ferran Nieto-Fabregat and Qian Zhu and Corinne Viv{\`e}s and Yunqin Zhang and Angela Marseglia and Fabrizio Chiodo and Michel Th{\'e}paut and Diksha Rai and Kulkarni, {Suvarn S.} and {di Lorenzo}, Flaviana and Antonio Molinaro and Roberta Marchetti and Franck Fieschi and Guozhi Xiao and Biao Yu and Alba Silipo",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Published by American Chemical Society.",

year = "2024",

month = feb,

day = "26",

doi = "10.1021/jacsau.3c00748",

language = "English",

volume = "4",

pages = "697--712",

journal = "JACS AU",

issn = "2691-3704",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes

AU - Nieto-Fabregat, Ferran

AU - Zhu, Qian

AU - Vivès, Corinne

AU - Zhang, Yunqin

AU - Marseglia, Angela

AU - Chiodo, Fabrizio

AU - Thépaut, Michel

AU - Rai, Diksha

AU - Kulkarni, Suvarn S.

AU - di Lorenzo, Flaviana

AU - Molinaro, Antonio

AU - Marchetti, Roberta

AU - Fieschi, Franck

AU - Xiao, Guozhi

AU - Yu, Biao

AU - Silipo, Alba

PY - 2024/2/26

Y1 - 2024/2/26

N2 - The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical dissection of BVMPK LPS components, attained by synthetic chemistry combined to spectroscopic, biophysical, and computational techniques, allowed to finely map the LPS epitopes recognized by DC-SIGN. Our findings reveal BVMPK’s role in immune modulation via DC-SIGN, targeting both the LPS O-antigen and the core oligosaccharide. Furthermore, when framed within medical chemistry or drug design, our results could lead to the development of tailored molecules to benefit the hosts dealing with inflammatory diseases.

AB - The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical dissection of BVMPK LPS components, attained by synthetic chemistry combined to spectroscopic, biophysical, and computational techniques, allowed to finely map the LPS epitopes recognized by DC-SIGN. Our findings reveal BVMPK’s role in immune modulation via DC-SIGN, targeting both the LPS O-antigen and the core oligosaccharide. Furthermore, when framed within medical chemistry or drug design, our results could lead to the development of tailored molecules to benefit the hosts dealing with inflammatory diseases.

KW - Bacteroides vulgatus lipopolysaccharide

KW - DC-SIGN

KW - NMR spectroscopy

KW - gut microbiota

KW - lectins

UR - http://www.scopus.com/inward/record.url?scp=85185569042&partnerID=8YFLogxK

U2 - 10.1021/jacsau.3c00748

DO - 10.1021/jacsau.3c00748

M3 - Article

C2 - 38425910

SN - 2691-3704

VL - 4

SP - 697

EP - 712

JO - JACS AU

JF - JACS AU

IS - 2

ER -

Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes

Abstract

Keywords

Access to Document

Other files and links

Cite this