Au/PANA/PVAc and Au/P(ANA-co-CNTA)/PVAc electrospun nanofibers as tyrosinase immobilization supports

Rana Golshaei, Zeliha Guler Gokce, Sayed Mehdi Ghoreishi, A. Sezai Sarac

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Au/poly anthranilic acid/poly vinyl acetate and Au/poly(anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline)/poly vinyl acetate nanofibers through electrospinning and their modification with covalent tyrosinase (Ty) immobilization was performed. It was realized by surface activation using N-(3-dimetylaminopropyl)-N-ethylcarbodimide hydrochloride/N-hydroxysuccinimide chemistry. Electrochemical impedance spectroscopy (EIS), FTIR–ATR, Raman spectroscopy, and bicinchoninic acid assay analyses demonstrated that Ty was stably and covalently bonded onto the nanofibers. Increase in surface roughness [atomic force microscopy (AFM)] and the presence of Cu atoms in the nanofiber composition after enzyme immobilization confirmed the Ty immobilization. The charge transfer resistances of the nanofibers decreased due to changes in the nanofiber surfaces after attachment of enzyme.

Original languageEnglish
Pages (from-to)658-668
Number of pages11
JournalInternational Journal of Polymeric Materials and Polymeric Biomaterials
Volume66
Issue number13
DOIs
Publication statusPublished - 2 Sept 2017
Externally publishedYes

Keywords

  • Atomic force microscopy
  • covalent immobilization
  • electrochemical impedance spectroscopy
  • tyrosinase

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