TY - JOUR
T1 - Au/PANA/PVAc and Au/P(ANA-co-CNTA)/PVAc electrospun nanofibers as tyrosinase immobilization supports
AU - Golshaei, Rana
AU - Guler Gokce, Zeliha
AU - Ghoreishi, Sayed Mehdi
AU - Sezai Sarac, A.
PY - 2017/9/2
Y1 - 2017/9/2
N2 - Au/poly anthranilic acid/poly vinyl acetate and Au/poly(anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline)/poly vinyl acetate nanofibers through electrospinning and their modification with covalent tyrosinase (Ty) immobilization was performed. It was realized by surface activation using N-(3-dimetylaminopropyl)-N-ethylcarbodimide hydrochloride/N-hydroxysuccinimide chemistry. Electrochemical impedance spectroscopy (EIS), FTIR–ATR, Raman spectroscopy, and bicinchoninic acid assay analyses demonstrated that Ty was stably and covalently bonded onto the nanofibers. Increase in surface roughness [atomic force microscopy (AFM)] and the presence of Cu atoms in the nanofiber composition after enzyme immobilization confirmed the Ty immobilization. The charge transfer resistances of the nanofibers decreased due to changes in the nanofiber surfaces after attachment of enzyme.
AB - Au/poly anthranilic acid/poly vinyl acetate and Au/poly(anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline)/poly vinyl acetate nanofibers through electrospinning and their modification with covalent tyrosinase (Ty) immobilization was performed. It was realized by surface activation using N-(3-dimetylaminopropyl)-N-ethylcarbodimide hydrochloride/N-hydroxysuccinimide chemistry. Electrochemical impedance spectroscopy (EIS), FTIR–ATR, Raman spectroscopy, and bicinchoninic acid assay analyses demonstrated that Ty was stably and covalently bonded onto the nanofibers. Increase in surface roughness [atomic force microscopy (AFM)] and the presence of Cu atoms in the nanofiber composition after enzyme immobilization confirmed the Ty immobilization. The charge transfer resistances of the nanofibers decreased due to changes in the nanofiber surfaces after attachment of enzyme.
KW - Atomic force microscopy
KW - covalent immobilization
KW - electrochemical impedance spectroscopy
KW - tyrosinase
UR - http://www.scopus.com/inward/record.url?scp=85018953954&partnerID=8YFLogxK
U2 - https://doi.org/10.1080/00914037.2016.1252360
DO - https://doi.org/10.1080/00914037.2016.1252360
M3 - Article
SN - 0091-4037
VL - 66
SP - 658
EP - 668
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
IS - 13
ER -