TY - JOUR
T1 - Bacterial deposition to fluoridated and non-fluoridated polyurethane coatings with different elastic modulus and surface tension in a parallel plate and a stagnation point flow chamber
AU - Bakker, Dewi P.
AU - Huijs, Frank M.
AU - De Vries, Joop
AU - Klijnstra, Job W.
AU - Busscher, Henk J.
AU - Van Der Mei, Henny C.
N1 - Funding Information: This work was supported by IOP milieutechnologie/Zware Metalen, Senter, The Netherlands.
PY - 2003/11/1
Y1 - 2003/11/1
N2 - Deposition of three marine bacterial strains with different cell surface hydrophobicities from artificial seawater to polyurethane coatings on glass with different surface tensions and elastic modulus was studied in situ in a parallel plate (PP) and stagnation point (SP) flow chamber. Different surface tensions of the coatings were established by changing the amount of fluorine, whereas using more or less branched polymers made different elastic moduli. Surface tensions of the coating, derived from measured contact angles with liquids, ranged from 11.9 to 44.9 mJ m-2, while the elastic moduli, derived from force-distance curves as measured with an atomic force microscope were between 1.5 and 2.2 GPa. In both flow chambers, the most hydrophilic bacterium Halomonas pacifica adhered preferentially to the more hydrophilic, non-fluoridated coating, whereas the most hydrophobic bacterium Marinobacter hydrocarbonoclasticus showed a greater preference for the more hydrophobic coating. Bacterial adhesion in the PP flow chamber was not influenced by the elastic modulus of the coatings, but in the SP flow chamber bacteria adhered in higher numbers to hard surfaces than to coatings of lower elastic moduli.
AB - Deposition of three marine bacterial strains with different cell surface hydrophobicities from artificial seawater to polyurethane coatings on glass with different surface tensions and elastic modulus was studied in situ in a parallel plate (PP) and stagnation point (SP) flow chamber. Different surface tensions of the coatings were established by changing the amount of fluorine, whereas using more or less branched polymers made different elastic moduli. Surface tensions of the coating, derived from measured contact angles with liquids, ranged from 11.9 to 44.9 mJ m-2, while the elastic moduli, derived from force-distance curves as measured with an atomic force microscope were between 1.5 and 2.2 GPa. In both flow chambers, the most hydrophilic bacterium Halomonas pacifica adhered preferentially to the more hydrophilic, non-fluoridated coating, whereas the most hydrophobic bacterium Marinobacter hydrocarbonoclasticus showed a greater preference for the more hydrophobic coating. Bacterial adhesion in the PP flow chamber was not influenced by the elastic modulus of the coatings, but in the SP flow chamber bacteria adhered in higher numbers to hard surfaces than to coatings of lower elastic moduli.
KW - Bacterial deposition
KW - Elastic modulus
KW - Flow chamber
KW - Marine bacteria
KW - Surface tension
UR - http://www.scopus.com/inward/record.url?scp=0142200385&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/S0927-7765(03)00159-0
DO - https://doi.org/10.1016/S0927-7765(03)00159-0
M3 - Article
SN - 0927-7765
VL - 32
SP - 179
EP - 190
JO - Colloids and surfaces B: Biointerfaces
JF - Colloids and surfaces B: Biointerfaces
IS - 3
ER -