Bromoperoxidase from Ascophyllum nodosum: a novel class of enzymes containing vanadium as a prosthetic group?

E. de Boer; Y. van Kooyk; M. G.M. Tromp; H. Plat; R. Wever

doi:https://doi.org/10.1016/0167-4838(86)90308-0

Bromoperoxidase from Ascophyllum nodosum: a novel class of enzymes containing vanadium as a prosthetic group?

E. de Boer, Y. van Kooyk, M. G.M. Tromp, H. Plat, R. Wever

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Reconstitution experiments showed that the transition metal vanadium is essential for enzymic activity of bromoperoxidase from the marine brown alga Ascophyllum nodosum. A linear relationship existed between brominating activity and the amount of vanadium incorporated in the enzyme. Maximal activity was found when the enzyme contained 5.1 nmol vanadium per mg bromoperoxidase. Based on gel-exclusion high-performance liquid chromatography, the molecular mass of bromoperoxidase was estimated as 90 000. Chemical analysis and electron paramagnetic resonance (EPR) demonstrated that bromoperoxidase contains vanadium (4.4 nmol per mg bromoperoxidase). This value corresponds to a ratio of 0.4 mol vanadium per mol bromoperoxidase. The EPR experiments suggest that in bromoperoxidase the vanadium ion is present in the 5 + redox state. These experiments demonstrate for the first time the existence of an enzyme containing vanadium at the active site.

Original language	English
Pages (from-to)	48-53
Number of pages	6
Journal	Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume	869
Issue number	1
DOIs	https://doi.org/10.1016/0167-4838(86)90308-0
Publication status	Published - 17 Jan 1986

Keywords

(A. nodosum)
Bromoperoxidase
ESR
Halometabolite
Prosthetic group
Vanadium

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title = "Bromoperoxidase from Ascophyllum nodosum: a novel class of enzymes containing vanadium as a prosthetic group?",

abstract = "Reconstitution experiments showed that the transition metal vanadium is essential for enzymic activity of bromoperoxidase from the marine brown alga Ascophyllum nodosum. A linear relationship existed between brominating activity and the amount of vanadium incorporated in the enzyme. Maximal activity was found when the enzyme contained 5.1 nmol vanadium per mg bromoperoxidase. Based on gel-exclusion high-performance liquid chromatography, the molecular mass of bromoperoxidase was estimated as 90 000. Chemical analysis and electron paramagnetic resonance (EPR) demonstrated that bromoperoxidase contains vanadium (4.4 nmol per mg bromoperoxidase). This value corresponds to a ratio of 0.4 mol vanadium per mol bromoperoxidase. The EPR experiments suggest that in bromoperoxidase the vanadium ion is present in the 5 + redox state. These experiments demonstrate for the first time the existence of an enzyme containing vanadium at the active site.",

keywords = "(A. nodosum), Bromoperoxidase, ESR, Halometabolite, Prosthetic group, Vanadium",

author = "{de Boer}, E. and {van Kooyk}, Y. and Tromp, {M. G.M.} and H. Plat and R. Wever",

note = "Funding Information: We thank H, Balke from the Laboratory of Analytical Chemistry, University of Amsterdam, for performing the ICAP-AES measurements. We gratefully acknowledge the support by DSM B.V., Geleen, The Netherlands. This work is part of the research programme of the Netherlands Foundation for Chemical Research (S.O.N,) and was made possible by financial support from the Netherlands Technology Foundation (S.T.W.).",

year = "1986",

month = jan,

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doi = "https://doi.org/10.1016/0167-4838(86)90308-0",

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T2 - a novel class of enzymes containing vanadium as a prosthetic group?

AU - de Boer, E.

AU - van Kooyk, Y.

AU - Tromp, M. G.M.

AU - Plat, H.

AU - Wever, R.

N1 - Funding Information: We thank H, Balke from the Laboratory of Analytical Chemistry, University of Amsterdam, for performing the ICAP-AES measurements. We gratefully acknowledge the support by DSM B.V., Geleen, The Netherlands. This work is part of the research programme of the Netherlands Foundation for Chemical Research (S.O.N,) and was made possible by financial support from the Netherlands Technology Foundation (S.T.W.).

PY - 1986/1/17

Y1 - 1986/1/17

N2 - Reconstitution experiments showed that the transition metal vanadium is essential for enzymic activity of bromoperoxidase from the marine brown alga Ascophyllum nodosum. A linear relationship existed between brominating activity and the amount of vanadium incorporated in the enzyme. Maximal activity was found when the enzyme contained 5.1 nmol vanadium per mg bromoperoxidase. Based on gel-exclusion high-performance liquid chromatography, the molecular mass of bromoperoxidase was estimated as 90 000. Chemical analysis and electron paramagnetic resonance (EPR) demonstrated that bromoperoxidase contains vanadium (4.4 nmol per mg bromoperoxidase). This value corresponds to a ratio of 0.4 mol vanadium per mol bromoperoxidase. The EPR experiments suggest that in bromoperoxidase the vanadium ion is present in the 5 + redox state. These experiments demonstrate for the first time the existence of an enzyme containing vanadium at the active site.

AB - Reconstitution experiments showed that the transition metal vanadium is essential for enzymic activity of bromoperoxidase from the marine brown alga Ascophyllum nodosum. A linear relationship existed between brominating activity and the amount of vanadium incorporated in the enzyme. Maximal activity was found when the enzyme contained 5.1 nmol vanadium per mg bromoperoxidase. Based on gel-exclusion high-performance liquid chromatography, the molecular mass of bromoperoxidase was estimated as 90 000. Chemical analysis and electron paramagnetic resonance (EPR) demonstrated that bromoperoxidase contains vanadium (4.4 nmol per mg bromoperoxidase). This value corresponds to a ratio of 0.4 mol vanadium per mol bromoperoxidase. The EPR experiments suggest that in bromoperoxidase the vanadium ion is present in the 5 + redox state. These experiments demonstrate for the first time the existence of an enzyme containing vanadium at the active site.

KW - (A. nodosum)

KW - Bromoperoxidase

KW - ESR

KW - Halometabolite

KW - Prosthetic group

KW - Vanadium

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Bromoperoxidase from Ascophyllum nodosum: a novel class of enzymes containing vanadium as a prosthetic group?

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Keywords

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