C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ T cells

Alexey A. Nabatov; Marein A. W. P. de Jong; Lot de Witte; Silvia Bulgheresi; Teunis B. H. Geijtenbeek

doi:https://doi.org/10.1016/j.virol.2008.05.025

C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ T cells

Alexey A. Nabatov, Marein A. W. P. de Jong, Lot de Witte, Silvia Bulgheresi, Teunis B. H. Geijtenbeek

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the structural homologue of DC-SIGN, the nematode C-type lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present on HIV-1 gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions

Original language	English
Pages (from-to)	323-328
Journal	Virology
Volume	378
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.05.025
Publication status	Published - 2008

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https://doi.org/10.1016/j.virol.2008.05.025

Cite this

@article{04566abded8f45dfb0332bc799bab749,

title = "C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ T cells",

abstract = "Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the structural homologue of DC-SIGN, the nematode C-type lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present on HIV-1 gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions",

author = "Nabatov, {Alexey A.} and {de Jong}, {Marein A. W. P.} and {de Witte}, Lot and Silvia Bulgheresi and Geijtenbeek, {Teunis B. H.}",

year = "2008",

doi = "https://doi.org/10.1016/j.virol.2008.05.025",

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issn = "0042-6822",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ T cells

AU - Nabatov, Alexey A.

AU - de Jong, Marein A. W. P.

AU - de Witte, Lot

AU - Bulgheresi, Silvia

AU - Geijtenbeek, Teunis B. H.

PY - 2008

Y1 - 2008

N2 - Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the structural homologue of DC-SIGN, the nematode C-type lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present on HIV-1 gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions

AB - Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading HIV-1 through the interaction of the gp120 oligosaccharides with the C-type lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to prevent interactions with DCs and subsequent viral transmission. Here, we have investigated whether the structural homologue of DC-SIGN, the nematode C-type lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data demonstrate that Mermaid interacts with high mannose structures present on HIV-1 gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have identified Mermaid as a non-cytotoxic agent that shares the glycan specificity with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 interactions

U2 - https://doi.org/10.1016/j.virol.2008.05.025

DO - https://doi.org/10.1016/j.virol.2008.05.025

M3 - Article

C2 - 18597806

SN - 0042-6822

VL - 378

SP - 323

EP - 328

JO - Virology

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