TY - JOUR
T1 - Calcium channel subunit α2σ4 is regulated by early growth response 1 and facilitates epileptogenesis
AU - van Loo, Karen M. J.
AU - Rummel, Christine K.
AU - Pitsch, Julika
AU - Müller, Johannes Alexander
AU - Bikbaev, Arthur F.
AU - Martinez-Chavez, Erick
AU - Blaess, Sandra
AU - Dietrich, Dirk
AU - Heine, Martin
AU - Becker, Albert J.
AU - Schoch, Susanne
PY - 2019/4/24
Y1 - 2019/4/24
N2 - Transient brain insults, including status epilepticus (SE), can trigger a period of epileptogenesis during which functional and structural reorganization of neuronal networks occurs resulting in the onset of focal epileptic seizures. In recent years, mechanisms that regulate the dynamic transcription of individual genes during epileptogenesis and thereby contribute to the development of a hyperexcitable neuronal network have been elucidated. Our own results have shown early growth response 1 (Egr1) to transiently increase expression of the T-type voltage-dependent Ca2+ channel (VDCC) subunit CaV3.2, a key proepileptogenic protein. However, epileptogenesis involves complex and dynamic transcriptomic alterations; and so far, our understanding of the transcriptional control mechanism of gene regulatory networks that act in the same processes is limited. Here, we have analyzed whether Egr1 acts as a key transcriptional regulator for genes contributing to the development of hyperexcitability during epileptogenesis.Wefound Egr1 to drive the expression of theVDCC subunitα2σ4, which was augmented early and persistently after pilocarpine-induced SE. Furthermore, we show that increasing levels of α2σ4 in the CA1 region of the hippocampus elevate seizure susceptibility of mice by slightly decreasing local network activity. Interestingly, we also detected increased expression levels of Egr1 andα2σ4 in human hippocampal biopsies obtained from epilepsy surgery. In conclusion, Egr1 controls the abundance of theVDCCsubunits CaV3.2 andα2σ4, which act synergistically in epileptogenesis, and thereby contributes to a seizure-induced “transcriptional Ca2+ channelopathy".
AB - Transient brain insults, including status epilepticus (SE), can trigger a period of epileptogenesis during which functional and structural reorganization of neuronal networks occurs resulting in the onset of focal epileptic seizures. In recent years, mechanisms that regulate the dynamic transcription of individual genes during epileptogenesis and thereby contribute to the development of a hyperexcitable neuronal network have been elucidated. Our own results have shown early growth response 1 (Egr1) to transiently increase expression of the T-type voltage-dependent Ca2+ channel (VDCC) subunit CaV3.2, a key proepileptogenic protein. However, epileptogenesis involves complex and dynamic transcriptomic alterations; and so far, our understanding of the transcriptional control mechanism of gene regulatory networks that act in the same processes is limited. Here, we have analyzed whether Egr1 acts as a key transcriptional regulator for genes contributing to the development of hyperexcitability during epileptogenesis.Wefound Egr1 to drive the expression of theVDCC subunitα2σ4, which was augmented early and persistently after pilocarpine-induced SE. Furthermore, we show that increasing levels of α2σ4 in the CA1 region of the hippocampus elevate seizure susceptibility of mice by slightly decreasing local network activity. Interestingly, we also detected increased expression levels of Egr1 andα2σ4 in human hippocampal biopsies obtained from epilepsy surgery. In conclusion, Egr1 controls the abundance of theVDCCsubunits CaV3.2 andα2σ4, which act synergistically in epileptogenesis, and thereby contributes to a seizure-induced “transcriptional Ca2+ channelopathy".
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065336425&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30792272
U2 - https://doi.org/10.1523/JNEUROSCI.1731-18.2019
DO - https://doi.org/10.1523/JNEUROSCI.1731-18.2019
M3 - Article
C2 - 30792272
SN - 0270-6474
VL - 39
SP - 3175
EP - 3187
JO - Journal of neuroscience
JF - Journal of neuroscience
IS - 17
ER -