TY - JOUR
T1 - Capillary blood microsampling to determine serum biopharmaceutical concentration: Mitra® microsampler vs dried blood spot
AU - Bloem, Karien
AU - Schaap, Tiny
AU - Boshuizen, Ronald
AU - Kneepkens, Eva L.
AU - Wolbink, Gerrit J.
AU - Vries, Annick De
AU - Rispens, Theo
PY - 2018
Y1 - 2018
N2 - Aim: For assessment of concentrations of biopharmaceuticals, for example, therapeutic drug monitoring, dried blood sampling of capillary blood is a convenient alternative to traditional venepuncture sampling. We investigated an alternative to dried blood spot collection on filter paper: sampling capillary blood using the Mitra® microsampler. Materials and Methods: Therapeutic monoclonal antibodies were spiked in whole blood, sampled using filter paper and Mitra microsampler and concentrations measured using specific ELISAs. Results: Good recoveries of adalimumab, infliximab, ustekinumab, vedolizumab, tocilizumab, natalizumab and rituximab were found up to 1 month of storage at room temperature, averaging 95.2% for the Mitra microsampler and 92.9% for Whatman® paper. Both hemoglobin and potassium yield satisfactory estimates for the volume of the cellular fraction of blood samples in combination with the Mitra microsampler. Conclusion: We established practical protocols for the estimation of serum/plasma concentrations of therapeutic antibodies via capillary blood microsampling.
AB - Aim: For assessment of concentrations of biopharmaceuticals, for example, therapeutic drug monitoring, dried blood sampling of capillary blood is a convenient alternative to traditional venepuncture sampling. We investigated an alternative to dried blood spot collection on filter paper: sampling capillary blood using the Mitra® microsampler. Materials and Methods: Therapeutic monoclonal antibodies were spiked in whole blood, sampled using filter paper and Mitra microsampler and concentrations measured using specific ELISAs. Results: Good recoveries of adalimumab, infliximab, ustekinumab, vedolizumab, tocilizumab, natalizumab and rituximab were found up to 1 month of storage at room temperature, averaging 95.2% for the Mitra microsampler and 92.9% for Whatman® paper. Both hemoglobin and potassium yield satisfactory estimates for the volume of the cellular fraction of blood samples in combination with the Mitra microsampler. Conclusion: We established practical protocols for the estimation of serum/plasma concentrations of therapeutic antibodies via capillary blood microsampling.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048742913&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/29863412
U2 - https://doi.org/10.4155/bio-2018-0010
DO - https://doi.org/10.4155/bio-2018-0010
M3 - Article
C2 - 29863412
SN - 1757-6180
VL - 10
SP - 815
EP - 823
JO - Bioanalysis
JF - Bioanalysis
IS - 11
ER -