TY - JOUR
T1 - Cardioprotection by Remote Ischemic Preconditioning is Blocked in the Aged Rat Heart in Vivo
AU - Behmenburg, Friederike
AU - Heinen, André
AU - Bruch, Lilli Vom
AU - Hollmann, Markus W.
AU - Huhn, Ragnar
PY - 2017
Y1 - 2017
N2 - Objectives: In animal studies, remote ischemic preconditioning (RIPC) is a powerful tool to protect the heart from ischemia and reperfusion injury. Unfortunately, this effect was not seen consistently in recent large clinical trials. Aging was shown to be a confounding factor for the effect of direct preconditioning in experimental studies, but whether aging also can influence the effect of RIPC and thus be responsible for the contradictory clinical effect is unknown. The aim of this study was to investigate whether the cardioprotective effect of RIPC was abolished by aging. Design: Randomized, prospective, blinded laboratory investigation. Setting: Experimental laboratory. Participants: Male Wistar rats. Interventions: Anesthetized young (Y, 2-3 months) and aged (A, 22-24 months) male Wistar rats were randomized to 4 groups (n = 6 per group). Control animals (Y-Con and A-Con) were not treated further; RIPC groups (Y-RIPC and A-RIPC) received 4 cycles of 5 minutes of bilateral hind limb ischemia interspersed with 5 minutes reperfusion before myocardial ischemia and reperfusion. All animals underwent 25 minutes of regional myocardial ischemia and 120 minutes of reperfusion. At the end of reperfusion, infarct size was determined by Tit staining. Measurements and Main Results: In the control group of young rats, infarct size was 56 +/- 9% of the area at risk. RIPC reduced infarct size to 31 +/- 9% (p <0.05 v Y-Con). Cardioprotection by RIPC was abolished completely in the aged rat heart (A-RIPC: 62 +/- 8%, A-Con: 63 +/- 4%; ns). Conclusions: The results of the authors' study showed that cardioprotection induced by remote ischemic preconditioning was blocked in the aged rat heart. (C) 2017 Elsevier Inc. All rights reserved
AB - Objectives: In animal studies, remote ischemic preconditioning (RIPC) is a powerful tool to protect the heart from ischemia and reperfusion injury. Unfortunately, this effect was not seen consistently in recent large clinical trials. Aging was shown to be a confounding factor for the effect of direct preconditioning in experimental studies, but whether aging also can influence the effect of RIPC and thus be responsible for the contradictory clinical effect is unknown. The aim of this study was to investigate whether the cardioprotective effect of RIPC was abolished by aging. Design: Randomized, prospective, blinded laboratory investigation. Setting: Experimental laboratory. Participants: Male Wistar rats. Interventions: Anesthetized young (Y, 2-3 months) and aged (A, 22-24 months) male Wistar rats were randomized to 4 groups (n = 6 per group). Control animals (Y-Con and A-Con) were not treated further; RIPC groups (Y-RIPC and A-RIPC) received 4 cycles of 5 minutes of bilateral hind limb ischemia interspersed with 5 minutes reperfusion before myocardial ischemia and reperfusion. All animals underwent 25 minutes of regional myocardial ischemia and 120 minutes of reperfusion. At the end of reperfusion, infarct size was determined by Tit staining. Measurements and Main Results: In the control group of young rats, infarct size was 56 +/- 9% of the area at risk. RIPC reduced infarct size to 31 +/- 9% (p <0.05 v Y-Con). Cardioprotection by RIPC was abolished completely in the aged rat heart (A-RIPC: 62 +/- 8%, A-Con: 63 +/- 4%; ns). Conclusions: The results of the authors' study showed that cardioprotection induced by remote ischemic preconditioning was blocked in the aged rat heart. (C) 2017 Elsevier Inc. All rights reserved
U2 - https://doi.org/10.1053/j.jvca.2016.07.005
DO - https://doi.org/10.1053/j.jvca.2016.07.005
M3 - Article
C2 - 27793521
SN - 1053-0770
VL - 31
SP - 1223
EP - 1226
JO - Journal of cardiothoracic and vascular anesthesia
JF - Journal of cardiothoracic and vascular anesthesia
IS - 4
ER -